Rabbit liver with coccidiosis

[Olympusman]

Healthy rabbit liver





Rabbit liver infected with coccidiosis





Detail showing protozoans



http://en.wikivet.net/index.php?title=C ... mat=mobile

http://en.wikipedia.org/wiki/Coccidiosis

[BJ]

Michael,


just a short message to thank you for all the histology posts. Your photos are beautifully clear and informative. Once upon a time many many years ago I was looking for a photo of this coccidiosis and could find only a very poor half out of focus black and white photo in a textbook...where were you when I needed you !!

thanks again,

Brian

[Olympusman]

Thanks, Brian.
As I have written before, I have been accumulating commercial histology slides for some time. If I ever get off my butt and get my microtome setup going there may be more.
A few notes on my techniques. I focus stack all of my histology micrographs. It addresses problems with focus field flatness and obtains focus through the thickness of the tissue section. Some slides yield better sharpness than others, mostly depending on the thickness of the section. Sometimes I find "stripes" in the section, indicating a notch in the edge of the microtome knife.
If the slide presents interesting pathology, I include links to informative articles. I have picked up quite a bit of information about pathology to have a greater understanding of what I am looking at and appreciate those who look forward to my histology postings.

[BJ]

hello Michael,

it would never occur to me to stack a thin section - just a mental block brought on by the term "thin section" ! Obviously though from your images, it is well worth considering in many cases. I sometimes found a corrugated effect from wax sections that had not been adequately relaxed by floating on warm water - in this case the "ripples" were parallel to the blade. The sections might be of even thickness, but did not lie flat on the slide.

Please keep the sections coming!

Brian

[Olympusman]

I have encountered some of those "rippled" slides in my collection. Even with attempting to stack, the rippling causes refraction through the section and I just have to give up on the slide.