Liver fluke

Images made through a microscope. All subject types.

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Olympusman
Posts: 5066
Joined: Sun Jan 15, 2012 12:31 pm

Liver fluke

Post by Olympusman »

I thought I would round up my set of human parasites (I'm sorry, but American Congress members won't fit on standard slides) with the liver fluke Fascioloides magna.
This cross section was 14mm -- too big for a 4X objective, so I did the total view by running the slide through my 35mm film scanner (sorry about the low resolution, but the detail shot is better).

Image

Detailed shot

Image

http://en.wikipedia.org/wiki/Fascioloides_magna
Michael Reese Much FRMS EMS Bethlehem, Pennsylvania, USA

Cactusdave
Posts: 1631
Joined: Tue Jun 09, 2009 12:40 pm
Location: Bromley, Kent, UK

Post by Cactusdave »

Stitching of multiple individual images can work well with a subject like this which is pretty flat. The result will be an image that shows the entire subject, but retains much more detail when zoomed into. X4 looks like it would be a good choice of objective, but you might try X6.3 for more detail. Advice on stitching microscope images is available here. http://www.quekett.org/resources/stack- ... tching.htm
Leitz Ortholux 1, Zeiss standard, Nikon Diaphot inverted, Canon photographic gear

Olympusman
Posts: 5066
Joined: Sun Jan 15, 2012 12:31 pm

Stitching

Post by Olympusman »

Thanks, Cactus Dave, It occured to me this morning that I could have tried using the Panorama function in my Olympus DSLR to do a panoramic stitch. I'll experiment with this in the future.
Michael Reese Much FRMS EMS Bethlehem, Pennsylvania, USA

Olympusman
Posts: 5066
Joined: Sun Jan 15, 2012 12:31 pm

Fluke redux

Post by Olympusman »

Cactus Dave,
Panoramic function in the Olympus digicams didn't work worth a #####.
However, the Microsoft Image Composite Editor rules!

Seven shots

Image

I recall a few years ago when I was at Olympus one of my buddies showed me a freehand stitch he did with MICE and my attitude was "That's nice." Now I am a true believer. What I love is that if you don't like the blend, you can go back to an individual shot and massage it in Photoshop and give it another try.
Michael Reese Much FRMS EMS Bethlehem, Pennsylvania, USA

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