Home-Made Mikropolychromar V. 4.0

Images made through a microscope. All subject types.

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Donw
Posts: 75
Joined: Tue Jul 26, 2011 2:05 am
Location: Seattle

Home-Made Mikropolychromar V. 4.0

Post by Donw »

I am including an exploded diagram of this version, for those of you who are wondering what this thing looks like.

Image

This version is much better, especially with small subjects, like Pleuronema. That part of my expectations was fulfilled.

This version also works... pretty well... with a 40X objective. Its performance is still somewhat lackluster, but I have a mod in mind that might help with that.

The smaller Axial iris has made the biggest difference. I was unable to build a 16 blade iris as I had hoped, so I wound up using a 5 blade iris instead. It works much better than I thought it would. I still think a 12 or 16 blade iris would work better...

I am going to build V 5.0... maybe next month. The biggest difference is that I will have the pieces lazer cut, which means I should be able to build my custom iris.

This video shows changes in the Marginal and Axial iris'. I've added text to explane. Sorry about the poor focus!

http://youtu.be/olSKS5Wiu3Q

This video shows the Marginal iris going from closed, to open, then back to 'viewing'. Special thanks to Mr. Colps in the starring role. I told him it wouldn't be an action shot, but he just had to bust a move or two.

http://youtu.be/svnJTDNJHdg

If anyone is interested, I am interested in building a copy for someone using a better microscope. And somebody who actually has photographic talent. This thing might work well for someone who knows what they are doing. :lol:

According to my research, plan achros are supposed to be the best, it is what Ziess sold with their Mikropolychromar. One refrence specifically said that fluors were 'a problem' with Rheinburg. I don't see why that would be, as they work pretty well with DF...

Anyway, if you would like to have one, please contact me.

Thanks for viewing.

Protos
Posts: 346
Joined: Tue Jan 07, 2014 4:01 am
Location: Lille, France

Post by Protos »

It looks interesting (for a ciliate fan like me) What does it look like on a photo ? There must need quite a lot of space. A good Idea might be to post before/after photos.

arturoag75
Posts: 1600
Joined: Fri Feb 05, 2010 1:05 am
Location: italy
Contact:

Post by arturoag75 »

Really very interesting :wink:

Donw
Posts: 75
Joined: Tue Jul 26, 2011 2:05 am
Location: Seattle

Post by Donw »

Protos wrote:It looks interesting (for a ciliate fan like me) What does it look like on a photo ? There must need quite a lot of space. A good Idea might be to post before/after photos.
My Abbe BF condenser is 48mm from the bottom of the filter holder to the top of the condenser. it is 35mm from the top of the mounting fork to the top of the condenser.

The MPK is 58mm from the bottom of the unit to the top of the Abbe condenser. It is 44mm from the top of the mounting fork to the top of the Abbe condenser, and 50mm from the top of the mounting fork to the top of the Aplanat condenser.

So... it is taller, but not hugely so. With the Abbe condenser on top, it will slide into the fork with no other adjustments. With the Aplanat on top, I have to raise the stage up about 10mm or so to slide it into the fork.

No biggie.

I'd give you a photo or two, but currently, I have no lens for my camera.

RogelioMoreno
Posts: 2962
Joined: Fri Nov 20, 2009 11:24 am
Location: Panama

Post by RogelioMoreno »

Interesting!

Rogelio

dolmadis
Posts: 883
Joined: Wed Dec 07, 2011 1:51 pm
Location: UK

Post by dolmadis »

Hi Don

I sent you a PM.

John

dolmadis
Posts: 883
Joined: Wed Dec 07, 2011 1:51 pm
Location: UK

Post by dolmadis »

Hi Don

Thanks for the reply by PM. Good to go !! I have just sent you another PM with my personal contact details.

John

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