Closterium: cell division in time lapse

myriophyllum · Post by **myriophyllum** » Sun Jan 19, 2014 7:52 am

Hi,

I'd like to show one more time lapse video now.
It is the third part of my little series of desmid divisions -
after Micrasterias and Euastrum here a Closterium:

http://www.youtube.com/watch?v=VtuBmXl0ttM

The cellular surrounding has in one scene been pointed out by putting some chinese ink into the water.

Illumination: Darkfield and asymmetric Rheinberg/Col mix
Zeiss Standard 18; Neofluar 16; Planapos 10, 25, 40 and 63; Ultrakondensor; Achr.-Apl. 1.4.
Time lapse (up to 250x) using the MagicLantern extention for Canon EOS.

My other desmid videos can be found here:

http://www.youtube.com/watch?v=0P7RQV51 ... tHW21-gW_3

Best wishes

Jens

fpelectronica · Post by **fpelectronica** » Sun Jan 19, 2014 8:07 am

Magnificent video

Tom Jones · Post by **Tom Jones** » Sun Jan 19, 2014 10:13 am

Jens,

Very nicely done!!

Was that done using the Magic Lantern RAW mode too?

Tom

myriophyllum · Post by **myriophyllum** » Sun Jan 19, 2014 10:20 am

Hi Tom,

no, RAW video doesn't work for Full HD on my 5DII, unfortunatally.

Thanks for your compliment

Jens

Tom Jones wrote:Jens,

Very nicely done!! Was that done using the Magic Lantern RAW mode too?

Tom

Jacek · Post by **Jacek** » Sun Jan 19, 2014 10:49 am

great job, beautiful video

Fredlab · Post by **Fredlab** » Sun Jan 19, 2014 11:06 am

Excellent works.
Thanks for sharing

myriophyllum · Post by **myriophyllum** » Mon Jan 20, 2014 4:03 am

Mercy Fred.

Salut

Jens

Fredlab wrote:Excellent works.
Thanks for sharing

arturoag75 · Post by **arturoag75** » Mon Jan 20, 2014 5:03 am

Superb work at all!

RogelioMoreno · Post by **RogelioMoreno** » Mon Jan 20, 2014 5:18 am

Interesting video.

Rogelio

carlos.uruguay · Post by **carlos.uruguay** » Mon Jan 20, 2014 5:38 am

Excellent!
As all your videos

myriophyllum · Post by **myriophyllum** » Tue Jan 21, 2014 6:58 am

Hi,

Thank You all for the nice compliments :-)

Greetings

Jens

Brian Matsumoto · Post by **Brian Matsumoto** » Tue Jan 21, 2014 11:29 am

I love videos that show a story and a theme. So your videos of the desmids are fascinating and satisfying for me to view. Your work is not only artistic, but it is also show great technical mastery over your equipment.

How do you make your slide mounts so that the plants can be maintained for these prolonged video sequences. Also, do you have any problems with focus drift when taking time lapse?

Brian Matsumoto

myriophyllum · Post by **myriophyllum** » Wed Jan 22, 2014 6:59 am

Thank You Brian,

I don't have much problems with focus shifts (more with the movement of the desmids...).
For constant distances between coverslip and slide I'm using Konstantan wire as spacer. This wire is available down to 0.04 mm diameter, here I used the 0.150 mm. From time to time it is necessary to replace evaporated water...
It helps to use large 24x40mm coverslips, too.

Best wishes

Jens

Brian Matsumoto wrote:I love videos that show a story and a theme. So your videos of the desmids are fascinating and satisfying for me to view. Your work is not only artistic, but it is also show great technical mastery over your equipment.

How do you make your slide mounts so that the plants can be maintained for these prolonged video sequences. Also, do you have any problems with focus drift when taking time lapse?

Brian Matsumoto

Closterium: cell division in time lapse

Closterium: cell division in time lapse

Konstantan spacers