Well, it's quite easy. I work on these litte creatures so I do it every day. Nematodes are really everywhere. You can extract them easily from any substrate. The extraction goes as follows:pwnell wrote:These are awesome. How did you get the worm to stay still? They always look like they are in epileptic shock when I try to photograph them.
You take a kind of pan to hold some water and put a mesh on it which is not touching the bottom of the pan. Put tissues or toilet paper on the mesh and then put your substrate you want on it. Add water until the tissue or toilet paper is just wet (not to much water because the nematodes go to the water. Let it, after a few hours the first nematodes are in the water. Remove the mesh from the pan. Sieve the water in the pan with a very fine sieve (about 40 µm mesh size) and observe under a stereomicroscope (transmitted light). Pick them with a fine needle (litte bit tricky the first time) and put them in a small drop on a microscope slide. Heat the drop with a lighter until the nematode lays still. Don't heat to long or all the water will evaporate. Then put a cover glass on it, seal it with nale polish and you can start to take pictures. The nematode will lay still.