Human Bone Cross Section

[Olympusman]

Human Bone thin cross section silver stained. Bone and nerve tissue are most commonly silver stained. I have some silver nitrate in my darkroom and want to find a formula for silver stain when I get my microtome up and running.

[Peter M. Macdonald]

Michael,

You could start with the Micortomist's Vade-mecum. The eighth edition is available on the Biodiversity Heritage Library.

Silver staining section starts at page 198.

Look forward to hearing how you get on.

Peter

[Pau]

Very clear image. The structure of the compact bone is very well shown.
Now the withe balance seems spot on.

[Olympusman]

I found a procedure for silver staining online.
First fix the section with formaldehyde or gluteraldehyde for a couple of days and then immerse the section in 2% silver nitrate for two days.
Pau, the color balance with the One-Touch White Balance is working well so far.

[Olympusman]

Found a download from the University of california, but at 600+ pages it is very unwieldy on a computer. However, I found a paperback print copy on Amazon for $16.50 plus tax. By the way, "Vade Mecum" is simply a highfaluten classical term for "manual."