Heliozoans - Scanning Electron Microscope images

Images made through a microscope. All subject types.

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Ecki
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Heliozoans - Scanning Electron Microscope images

Post by Ecki »

Heliozoans are the microbes that fascinated me most when I started with microscopy. Besides the well known larger heliozoans there are many very small species (10 µm diameter) that are nearly impossible to identify with a light microscope. Some of these small heliozoans reveal an amazing hidden beauty under the electron microscope.

These tiny heliozoans have axiopoda that get lost during preparation as well as siliceous scales. There are two typos of scales, tangential plate scales and radial scales. The tangential scales are usually much smaller.

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Pterocystis pulchra, periplast with radial scales


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Pterocystis pulchra, periplast with radial scales


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Pterocystis pulchra, periplast with radial scales and tangential scales


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Pterocystis pulchra, radial scales


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Pterocystis pulchra, radial scales


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Pterocystis striata, periplast with radial scales


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Pterocystis striata, radial scales


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Pseudoraphidiophrys formosa (=Pterocystis formosa), periplast with radial scales and tangential scales

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Pseudoraphidiophrys formosa, radial scales and tangential scales


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Pseudoraphidiophrys discoidea ( =Pterocystis discoidea), periplast with radial scales


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Pseudoraphidiophrys discoidea, periplast with radial scales and tangential scales


Some of the radial scales remind me of flowers. It is quite astonishing how nature produces beauty even at the smallest scale.

These heliozoans used to belong to the genus Acanthocystis. Then they were moved to a distinct new genus Pterocystis (Ptero = wing, as theses scales show a wing-like profile in the light microscope). The species P. discoidea und P. formosa were subsequently moved further into the genus Pseudoraphidiophrys. Of course my identification is not 100% as we know very little about these heliozoans and there is very little published literature.

I wish you all a great 2014 with many interesting subjects under the microscope.

Best regards,
Ecki

Jacek
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Post by Jacek »

great :shock:

Pau
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Post by Pau »

Really impressive!!
Are those scales visibles (even barely) with light microscopy?
Pau

Litonotus
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Post by Litonotus »

great, really impressive detail and atmosphere. Could you tell something about magnification used here?
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I'm looking for the the extemely rare V-IM magnification changer for the E800 scope. If you have seen a listing or have one for sale please let me know.

Ecki
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Post by Ecki »

Thank you all!
Are those scales visibles (even barely) with light microscopy?
Here is a link to Ferrys website: http://www.arcella.nl/pterocystis-spec

These are dried species in phase contrast. I don't think that these scales are visible normally.

The detail images are approximately 100.000x magnification, depends on your screen size :D

Litonotus
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Post by Litonotus »

thanks (:

light microsopy can do nothing here...
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I'm looking for the the extemely rare V-IM magnification changer for the E800 scope. If you have seen a listing or have one for sale please let me know.

arturoag75
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Post by arturoag75 »

:shock: :shock: impressive and beautiful details hidden into Heliozoan wow..
Thanks for posting :wink:
Arturo

g4lab
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Post by g4lab »

Congratulations on having the new baby up and running!!!

flyer2o12
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Post by flyer2o12 »

Hi Litonotus,

Which SEM are you using?

Marek Mis
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Post by Marek Mis »

Incredible images ! The structures look like leaves of some plant.

Marek

Ecki
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Post by Ecki »

Thank you, Marek.

Here I have two light microscopic images my friend Steffen made. He took the sample from a swamp and sent me half of it. We both prepared petri dishes from which I made my SEM preparations. When I find something interesting, he looks for the same species with a light microscope.

Here you have two images of Pterocystis with DIC:

Image

Image

On the last image Synura is present, too. The images were made for reference in pretty thick samples.

Best regards
Ecki

Litonotus
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Post by Litonotus »

I wonder how olympus 100/1,7 would perform... Or zeiss or olympus 150/1,35x objectives
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I'm looking for the the extemely rare V-IM magnification changer for the E800 scope. If you have seen a listing or have one for sale please let me know.

leonardturner
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Post by leonardturner »

Beautiful execution with a difficult instrument. Thank you!

pwnell
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Post by pwnell »

Please keep these coming. I love peeking deeper into the micro universe. If I had the money I'd get a SEM too and abscond from my day job.

Stefano B
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Post by Stefano B »

Hi Ecki,

which SEM are you using? Can you post some pictures of this microscope?

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