Recently I've got a sample from sphagnum ponds of alpine region of Austria. There are lots of desmids and I'm working to combine manually pictures because contrast is too weak for software-stacking. In this case I also unsed manual stitching because this banana was too long for the 63/1.4 objective. But only this objective shows precisely the surface structure of Closterium costata, i. e. the longitudinal stripes. You see also transversal stripes. Each of these indicates a cell division, so this Specimen had 11 divisions.
Closterium costatum
63/1,4, DIC
3W Luxeon
24 shots stacked and stitched manually
Olympus C7070
Closterium bears star shaped chloroplasts with pyrenoid bodies in the center. This picture showes the pyrenoid layer (axial circular structures).
63/1,4, DIC
3W Luxeon
6 shots stacked and stitched manually
Olympus C7070
Cheers, Wolfgang