My first posting: video link paramecium

Brian Matsumoto · Post by **Brian Matsumoto** » Tue Nov 12, 2013 10:38 am

Hello all:

I have been lurking in the background of this site, enjoying the spectacular images presented by the group members. I decided to try and join in the posting by providing a URL link to my video.

This is a phase contrast image of paramecia made with a Leitz Ortholux microscope and a color Heine condenser.

http://www.youtube.com/watch?v=TFsvMQYe-E8

Please let me know what you think.

Thanks

Brian

Pau · Post by **Pau** » Tue Nov 12, 2013 10:54 am

Brian, wellcome to the forum!
Very nice video, and pretty unusual equipment.

Cactusdave · Post by **Cactusdave** » Tue Nov 12, 2013 12:24 pm

"A color Heine condenser."

Wow, those are as rare as hen's teeth! Congratulations on actually owning one. The results are pretty interesting as well.

RogelioMoreno · Post by **RogelioMoreno** » Wed Nov 13, 2013 4:42 am

Very nice video.

Rogelio

Jacek · Post by **Jacek** » Wed Nov 13, 2013 2:26 pm

Hi Brian, nice video

pwnell · Post by **pwnell** » Wed Nov 13, 2013 3:46 pm

I agree, very unusual but striking.

Charles Krebs · Post by **Charles Krebs** » Wed Nov 13, 2013 10:42 pm

Welcome Brian... nice to see you here.

Very nice video, and certainly a lighting effect not often seen (a first for me at least).

carlos.uruguay · Post by **carlos.uruguay** » Fri Nov 15, 2013 4:59 am

Welcome.
Excellent video.
How does it achieve the colors in phase?
Excuse my bad english.
The fault is of google traslator
carlos - uruguay

Brian Matsumoto · Post by **Brian Matsumoto** » Fri Nov 15, 2013 11:07 am

Carlos:

The color Heine is an unusual phase contrast.condenser. It throws up two concentric annuli of light: the obliquity of each cones can be adjusted by the operator. The inner annulus has a green filter and the cone's obliquity is adjusted until it matches the phase ring of the objective. The outer cone has a red filter and this is expanded until its light is not directly collected by the objective. Essentially this cone generates darkfield illumination.

As a result, the illumination is a combination of phase contrast and darkfield: the two lighting modalities are reflected by their colors. The green is the phase image and the red is the darkfield image. If an object has both phase and darkfield characteristics, then it is colored yellow.

According to John Field who is an expert on older Leitz microscopes, only 300 of the color Heine condensers were ever made.

Brian

carlos.uruguay · Post by **carlos.uruguay** » Fri Nov 15, 2013 11:22 am

Thanks Brian for your valuable information!