Damselfly nymph

Images made through a microscope. All subject types.

Moderators: rjlittlefield, ChrisR, Chris S., Pau

Charles Krebs
Posts: 5865
Joined: Tue Aug 01, 2006 8:02 pm
Location: Issaquah, WA USA
Contact:

Damselfly nymph

Post by Charles Krebs »

Here are a couple of shots of a fairly small damselfly nymph.

The first shot is a ventral view showing the multiple-hinged, extendable lower jaw (similar to that seen in dragonfly nymphs). Here it is in the "closed" position but it is thrust out quickly to grasp prey that might otherwise seem out of reach.

The second, lower magnification shot, is a view from above showing the trachea tubes branching through the body.

Nikon MM-11 microscope. Olympus MPlanFL N 10/0.30 objective. Olympus U-SWTR-3. Canon T3i camera.
Image


Nikon MM-11 microscope. Olympus MPlanFL N 5/0.15 objective. Olympus U-SWTR-3. Canon T3i camera.
Image
Last edited by Charles Krebs on Thu Nov 14, 2013 12:52 pm, edited 1 time in total.

Saul
Posts: 1767
Joined: Mon Jan 31, 2011 11:59 am
Location: Naperville, IL USA
Contact:

Post by Saul »

Excellent photos, especially second one ! What technique and setup was used ?
Thanks!

Jacek
Posts: 5354
Joined: Sun Oct 02, 2011 7:00 am
Location: Poland

Post by Jacek »

:shock: :smt038 :shock:

pwnell
Posts: 2029
Joined: Fri Dec 18, 2009 4:59 pm
Location: Tsawwassen, Canada

Post by pwnell »

Beautiful. Your dark field came out very nice - mine suffered from halos.

leonardturner
Posts: 701
Joined: Thu Mar 14, 2013 11:40 am
Location: Atlanta, GA, USA

Post by leonardturner »

Beautiful work, as always. pwnell's question is interesting; how do you avoid halos, is it equipment or technique?

RogelioMoreno
Posts: 2962
Joined: Fri Nov 20, 2009 11:24 am
Location: Panama

Post by RogelioMoreno »

Beautiful as always!

Rogelio

Olympusman
Posts: 4890
Joined: Sun Jan 15, 2012 12:31 pm

Darkfield

Post by Olympusman »

Depending on the size of the disks in my home made darkfield stops, I find I can stop down the condenser iris to some degree before the image blacks out. This reduces the amount of flare in the image.
Michael Reese Much FRMS EMS Bethlehem, Pennsylvania, USA

Sumguy01
Posts: 1687
Joined: Mon Jan 28, 2013 11:05 pm
Location: Ketchikan Alaska USA

Post by Sumguy01 »

=D> These are amazing .
Thanks for sharing .

Charles Krebs
Posts: 5865
Joined: Tue Aug 01, 2006 8:02 pm
Location: Issaquah, WA USA
Contact:

Post by Charles Krebs »

What technique and setup was used ?
Simple darkfield. The microscope and stage I used are like the second picture here:
http://www.photomacrography.net/forum/v ... hp?t=17656
(But the "tubes" between the microscope and camera body have been replaced with a super-widefield trinocular head).

The condenser is an inexpensive Chinese made stereo darkfield condenser that I mounted on top of an eBay "Right Angle Spy Scope". This was positioned under the stage. A fiber optic light guide is positioned to shine into the angled mirror for the light.
Image

The subject was in a home-made "well" slide, which is nothing more than a plumbing washer glued to a 2x3 inch microscope slide.

I have no magical way of avoiding halos in darkfield stacks, they are a problem for me as well. They are far less of a problem with lower magnifications (such as 5X and 10X here) and also in cases where the subject is rather flat. The legs here are actually not that 3-dimensional and positioned parallel to the camera back so they were not too much of a problem. (Not to say there was not some PS clean-up... there was. :wink: )

Saul
Posts: 1767
Joined: Mon Jan 31, 2011 11:59 am
Location: Naperville, IL USA
Contact:

Post by Saul »

Thanks Charles, it was very useful ! :idea:
Did you try to use a flash instead fiber ?

arturoag75
Posts: 1600
Joined: Fri Feb 05, 2010 1:05 am
Location: italy
Contact:

Post by arturoag75 »

Fantastic job , as usual :wink:
Arturo

carlos.uruguay
Posts: 5356
Joined: Thu Feb 23, 2012 8:05 pm
Location: Uruguay - Montevideo - America del Sur
Contact:

Post by carlos.uruguay »

Without words!

leonardturner
Posts: 701
Joined: Thu Mar 14, 2013 11:40 am
Location: Atlanta, GA, USA

Post by leonardturner »

Very much appreciate your sharing your experience and techniques; it has been a great help!

Olympusman
Posts: 4890
Joined: Sun Jan 15, 2012 12:31 pm

darkfield

Post by Olympusman »

Dang, Charlie -- that's a crazy setup. Do you have a post for the rht angle gizmo? It looks pretty small.
Michael Reese Much FRMS EMS Bethlehem, Pennsylvania, USA

Post Reply Previous topicNext topic