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Images made through a microscope. All subject types.

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curt0909
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Post by curt0909 »

The excellent images coming from Rogelio and Waldo have inspired me to try some more fluorescence illumination. Blue excitation- green red emission. auttofluorescence. Small ant. 4x objective. Color enhancements in post

Image

Image

Image

Image

JH
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Post by JH »

Made my day :)
Jörgen

harisA
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Post by harisA »

Very nice images i prefer first one.How many images in the stack?

pwnell
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Post by pwnell »

First one is really psychedelic! Care to post the original before post processing? Would be nice to compare.

Fredlab
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Post by Fredlab »

pwnell wrote:First one is really psychedelic! Care to post the original before post processing? Would be nice to compare.
+1

You rock.
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Jacek
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Post by Jacek »

:shock: :shock: :shock: :shock: :shock:

arturoag75
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Post by arturoag75 »

Very nice :wink:

RogelioMoreno
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Post by RogelioMoreno »

Very nice!

Rogelio

Pau
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Post by Pau »

Excellent image, something unique.
It seems a coloured SEM. In fact playing with levels and converting it to BN it seems an original SEM monochrome.

Please let us kow about your light source, illumination setup and filters used to take it.
Pau

Charles Krebs
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Post by Charles Krebs »

Impressive image!

Like Pau I wold like to know a little more about the process used (especially PP)

discomorphella
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Post by discomorphella »

Spectacular. And I 4th the motion to learn more about the optical and post-processing conditions. Great shots. Now all of the ants in my woods are in danger of visiting my microscope instead of being ignored....

David

curt0909
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Location: Pittsburgh, PA

Post by curt0909 »

Thanks for the comments. Here is the original image:Image

Vanox AHB3T microscope. Blue excitation fluorescence. 200w hg bulb. Zeiss Planapo 4x 0.14 160mm/- objective. Direct projection to canon t3i. 54 photos stacked with Zerene PMAX method. I never do this but for some reason I used 200 ISO and I think the image quality suffered from that.

In post i used smart sharpen, a simple mask with a steep curve nearly blacked out the background and left the hairs alone. A separate mask for the eye to alter the color. And one more tedious mask for the hairs. This one probably took 10 minutes to finish. l didn't complete the body, partly because they were difficult to distinguish.

The inverted photo(white background) did not have the hairs masked. Just the eye.

carlos.uruguay
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Post by carlos.uruguay »

Wonderful!!!!

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