Pick your flavor

curt0909 · Post by **curt0909** » Sun Oct 06, 2013 8:15 am

The excellent images coming from Rogelio and Waldo have inspired me to try some more fluorescence illumination. Blue excitation- green red emission. auttofluorescence. Small ant. 4x objective. Color enhancements in post

JH · Post by JH » Sun Oct 06, 2013 9:32 am

Made my day

Jörgen

harisA · Post by **harisA** » Sun Oct 06, 2013 10:39 am

Very nice images i prefer first one.How many images in the stack?

pwnell · Post by **pwnell** » Sun Oct 06, 2013 10:51 am

First one is really psychedelic! Care to post the original before post processing? Would be nice to compare.

Fredlab · Post by **Fredlab** » Sun Oct 06, 2013 11:05 am

pwnell wrote:First one is really psychedelic! Care to post the original before post processing? Would be nice to compare.

+1

You rock.

Jacek · Post by **Jacek** » Sun Oct 06, 2013 12:09 pm

arturoag75 · Post by **arturoag75** » Mon Oct 07, 2013 12:25 am

Very nice

RogelioMoreno · Post by **RogelioMoreno** » Mon Oct 07, 2013 4:42 am

Very nice!

Rogelio

Pau · Post by **Pau** » Mon Oct 07, 2013 8:51 am

Excellent image, something unique.
It seems a coloured SEM. In fact playing with levels and converting it to BN it seems an original SEM monochrome.

Please let us kow about your light source, illumination setup and filters used to take it.

Charles Krebs · Post by **Charles Krebs** » Mon Oct 07, 2013 2:54 pm

Impressive image!

Like Pau I wold like to know a little more about the process used (especially PP)

discomorphella · Post by **discomorphella** » Mon Oct 07, 2013 4:09 pm

Spectacular. And I 4th the motion to learn more about the optical and post-processing conditions. Great shots. Now all of the ants in my woods are in danger of visiting my microscope instead of being ignored....

David

curt0909 · Post by **curt0909** » Tue Oct 08, 2013 3:26 am

Thanks for the comments. Here is the original image:

Vanox AHB3T microscope. Blue excitation fluorescence. 200w hg bulb. Zeiss Planapo 4x 0.14 160mm/- objective. Direct projection to canon t3i. 54 photos stacked with Zerene PMAX method. I never do this but for some reason I used 200 ISO and I think the image quality suffered from that.

In post i used smart sharpen, a simple mask with a steep curve nearly blacked out the background and left the hairs alone. A separate mask for the eye to alter the color. And one more tedious mask for the hairs. This one probably took 10 minutes to finish. l didn't complete the body, partly because they were difficult to distinguish.

The inverted photo(white background) did not have the hairs masked. Just the eye.

carlos.uruguay · Post by **carlos.uruguay** » Tue Oct 08, 2013 8:13 am

Wonderful!!!!