Conochilus unicornis

Images made through a microscope. All subject types.

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Charles Krebs
Posts: 5865
Joined: Tue Aug 01, 2006 8:02 pm
Location: Issaquah, WA USA

Post by Charles Krebs »

Thanks all for the comments!

I especially like the two first ones. Are they taken with BF oblique illumination ?
Yes, the first two were oblique brightfield (with 10X), and primarily intended to give some idea of the spherical (here round) colony before I went in close with higher power objectives.

where did you find them, I would love to discover some of these in my pond.
Glad you asked... it gives me a reason to fire up Google Earth, one of my favorite... and time consuming (wasting? :wink:)... applications. These were found in a mass of free floating vegetation along the south shore of Lake Sammamish (a pretty large lake... 7 miles long by 1.5 miles) right where Issaquah Creek enters the lake. The vegetation was not rooted, but had been blown against the shore by a light wind. I collected a jar-full and was very pleasantly surprised to find dozens of these colonies.

I assume from the spectacular rendition of the cilia on the last three (higher mag) shots that you had these very well squashed down.
Sort of, but not exactly. ( :smt017 ) To me one of the really fascinating aspects of rotifers is the often seen metachronal wave action of the cilia. If you flatten them down too much the cilia starts to hit the against the cover-glass, and lose that wonderful aspect of the sequential motion. So the colony is flattened a bit, but not so much that there were not plenty of individuals to be photographed where the cilia is very close to, but not "beating" on the glass. In image 4 and 5 (20X) 6 (40X) and 7 (60X) it was possible to show this natural action. In the last image you can see that some of the cilia is against the glass (to the right of the eye-spot) so some areas of the cilia show the very pronounced "wave motion" while others are "flat". I kept the cover-slip "elevated" more than normal by including a small branched piece of vegetation that appeared to be about the appropriate thickness to accomplish this task.

did you ever consider giving a workshop?
I've toyed with the idea of doing a live "webinar", but just don't know it there is a large enough "community" of interested people to warrant it. If it ever develops into more than a "thought" I'll let you know.

Posts: 54
Joined: Sun Oct 09, 2011 10:44 am
Location: San Jose, CA

Post by uimike »

I am quite sure you would have a sizable audience!!

BTW, cool to see how you are using Earth to geolocate the sampling points.


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