Auto Fluorescence + 3D Deconvolution - The Beauties (part 2)

RogelioMoreno · Post by **RogelioMoreno** » Wed Sep 25, 2013 3:58 am

The following are my favorites amongs all my fluorescence stack with 3D deconvolution.

Pediastrum sp. by Rogelio Moreno G., on Flickr

Micrasterias furcata by Rogelio Moreno G., on Flickr

Micrasterias rotata by Rogelio Moreno G., on Flickr

Micrasterias rotata by Rogelio Moreno G., on Flickr

Hope you enjoy them!

Rogelio

Jacek · Post by **Jacek** » Wed Sep 25, 2013 4:21 am

Rogelio great, amazing. Congratulations

arturoag75 · Post by **arturoag75** » Wed Sep 25, 2013 6:24 am

Impressive

Ernst Hippe · Post by **Ernst Hippe** » Wed Sep 25, 2013 6:59 am

Rogelio,
I like the first one, it is Pediastrum duplex var. duplex, wellknown also here. Thanks for your info about Micrasterias laticeps!
Ernst

discomorphella · Post by **discomorphella** » Wed Sep 25, 2013 7:32 am

The Pediastrum is spectacular (so are the others but P. duplex is one of my favorite desmids).

David

Olympusman · Post by **Olympusman** » Wed Sep 25, 2013 8:10 am

Love the Desmids, especially the Pediastrum.

Ernst Hippe · Post by **Ernst Hippe** » Wed Sep 25, 2013 8:50 am

Michael,
we share the same love... but Pediastrum is not a desmid, but a green alga.

g4lab · Post by **g4lab** » Wed Sep 25, 2013 9:45 am

Rogelio can you show is the same image with and without deconvolution so that we can see what it does to the image?

Wonderful images!

RogelioMoreno · Post by **RogelioMoreno** » Thu Sep 26, 2013 7:07 am

Thank you all for your comments.

Gene, I open a new thread about the differences between deconvolve and not fluresce stack:

http://www.photomacrography.net/forum/v ... 887#134887

Rogelio

pwnell · Post by **pwnell** » Fri Sep 27, 2013 10:25 am

What is the difference between the fourth and fifth images? Different processing? cellSens vs Autoquant?

RogelioMoreno · Post by **RogelioMoreno** » Fri Sep 27, 2013 10:39 am

pwnell wrote:What is the difference between the fourth and fifth images? Different processing? cellSens vs Autoquant?

Good question, different time (and a little exposure time; but I do not think it will cause that differences), the stack of #4 was taken then later #5 (after two more stacks on the same specimen), I also noted the differences and posted them to see if someone could noted it (you have good eye), maybe the expose to ultraviolet light (two stacks between them) changed something.

Rogelio