Auto Fluorescence + 3D Deconvolution - The Beauties (part 2)

Images made through a microscope. All subject types.

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RogelioMoreno
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Location: Panama

Auto Fluorescence + 3D Deconvolution - The Beauties (part 2)

Post by RogelioMoreno »

The following are my favorites amongs all my fluorescence stack with 3D deconvolution.

Image
Pediastrum sp. by Rogelio Moreno G., on Flickr

Image
Micrasterias furcata by Rogelio Moreno G., on Flickr

Image
Micrasterias rotata by Rogelio Moreno G., on Flickr

Image
Micrasterias rotata by Rogelio Moreno G., on Flickr

Image
Micrasterias rotata by Rogelio Moreno G., on Flickr

Hope you enjoy them!

Rogelio

Jacek
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Post by Jacek »

Rogelio great, amazing. Congratulations

arturoag75
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Contact:

Post by arturoag75 »

Impressive :wink: :wink:

Ernst Hippe
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Location: Germany

Post by Ernst Hippe »

Rogelio,
I like the first one, it is Pediastrum duplex var. duplex, wellknown also here. Thanks for your info about Micrasterias laticeps!
Ernst

discomorphella
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Post by discomorphella »

The Pediastrum is spectacular (so are the others but P. duplex is one of my favorite desmids).

David

Olympusman
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Desmids

Post by Olympusman »

Love the Desmids, especially the Pediastrum.
Michael Reese Much FRMS EMS Bethlehem, Pennsylvania, USA

Ernst Hippe
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Joined: Sun Aug 16, 2009 9:41 am
Location: Germany

Post by Ernst Hippe »

Michael,
we share the same love... but Pediastrum is not a desmid, but a green alga.

g4lab
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Post by g4lab »

Rogelio can you show is the same image with and without deconvolution so that we can see what it does to the image?

Wonderful images!

RogelioMoreno
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Joined: Fri Nov 20, 2009 11:24 am
Location: Panama

Post by RogelioMoreno »

Thank you all for your comments.

Gene, I open a new thread about the differences between deconvolve and not fluresce stack:

http://www.photomacrography.net/forum/v ... 887#134887


Rogelio

pwnell
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Post by pwnell »

What is the difference between the fourth and fifth images? Different processing? cellSens vs Autoquant?

RogelioMoreno
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Joined: Fri Nov 20, 2009 11:24 am
Location: Panama

Post by RogelioMoreno »

pwnell wrote:What is the difference between the fourth and fifth images? Different processing? cellSens vs Autoquant?
Good question, different time (and a little exposure time; but I do not think it will cause that differences), the stack of #4 was taken then later #5 (after two more stacks on the same specimen), I also noted the differences and posted them to see if someone could noted it (you have good eye), maybe the expose to ultraviolet light (two stacks between them) changed something.

Rogelio

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