Auto Fluorescence + 3D Deconvolution - The Beauties (part 1)

Images made through a microscope. All subject types.

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RogelioMoreno
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Auto Fluorescence + 3D Deconvolution - The Beauties (part 1)

Post by RogelioMoreno »

Here are the first group of the beauties.

All images were done with the Plan Apo 20x/0.75 and the UV-2B cube with auto fluorescence. You have to do the stack quicly because the specimen auto-fluorescence get dim with the pass of the time.


Image
Closterium by Rogelio Moreno G., on Flickr

Image
Closterium by Rogelio Moreno G., on Flickr

Image
Micrasterias laticeps by Rogelio Moreno G., on Flickr

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Micrasterias laticeps by Rogelio Moreno G., on Flickr

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Micrasterias furcata by Rogelio Moreno G., on Flickr

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Micrasterias radiosa by Rogelio Moreno G., on Flickr

Rogelio
Last edited by RogelioMoreno on Tue Sep 24, 2013 4:24 am, edited 1 time in total.

Fredlab
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Post by Fredlab »

arf...

speechless
:shock:
I apologise for my poor english
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Cactusdave
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Post by Cactusdave »

Fascinating and beautiful results. Alas this could be yet another new way to drain the wallets of long suffering photomicrographers! :wink:
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Marek Mis
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Post by Marek Mis »

Rogelio,

Really spectacular images ! All of them are beautiful but my favourite is the 4 th one.
Thanks for sharing.

Marek

Jacek
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Post by Jacek »

beautiful :shock:

Ernst Hippe
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Post by Ernst Hippe »

Rogelio,
phantastic pictures of my beloved desmids!
The M.laticeps is not common over here - I got it only once from a supplier of tropical water plants, and only the variation with single tips at the side lobes. Where from did you get it and which variation(s)?
Regards Ernst

Ecki
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Post by Ecki »

Rogelio,

this is amazing. I think I need deconvolution software as well ;) What package did you use?

Best regards
Ecki

discomorphella
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Post by discomorphella »

Beautiful results. Now you need to figure out which antifade reagents are the least damaging to algae. You might try some dilute p-Phenylenediamine or DABCO (start with perhaps <= 20 micromolar in the water you use to mount the specimens and work up to larger concentrations if required) to try and reduce the photobleaching. Deconvolution results look great, can you please include some details as to which algorithm, how many slices, which model parameters etc that you are using?

David

Pau
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Post by Pau »

Extraordinary quality, both this ones and your Spirogyra.
Pau

Litonotus
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Post by Litonotus »

I'm sure we will see one of them in top 10 of small world or bioscapes (: great!
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pwnell
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Post by pwnell »

4th one is in a class of its own.

arturoag75
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Post by arturoag75 »

Really stunning and fascinating imsges.........
I' d like to know more on this tecnic :shock:

sebba28
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Post by sebba28 »

Stunning pictures, :shock:

RogelioMoreno
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Post by RogelioMoreno »

Thank you for your comments.

Ernst, I am getting my samples from lake Miraflores (this lake has some degree of salt because it is a reservoir for the Panama canal). If the specimen on picture #4 has double tips on the side lobes then I am finding single and double.

Ecki, I am using the 3o days trial of cellSens and AutoQuant X3.

David thank you for the tips about the p-Phenylenediamine, do you know where I can get it?

I am using the default settings on both cellSens and AutoQuant 3D deconvolution, no SA detection.

#1: 2322 x 1179 x 24 slides
#2: 2498 x 1339 x 14 slides
#3: 1851 x 1750 x 20 slides
#4: 1684 x 1688 x 23 slides
#5: 2061 x 2010 x 21 slides
#6: 2161 x 2073 x 16 slides

All with the Plan Apo 20x/0.75 and 1 um step.

Rogelio

Ecki
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Post by Ecki »

Hi Rogelio,

I looked at AutoQuant X3 and was very impressed. Do you know how much it costs?

Regards
Ecki

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