Auto Fluorescence + 3D Deconvolution - The Beauties (part 1)
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Auto Fluorescence + 3D Deconvolution - The Beauties (part 1)
Here are the first group of the beauties.
All images were done with the Plan Apo 20x/0.75 and the UV-2B cube with auto fluorescence. You have to do the stack quicly because the specimen auto-fluorescence get dim with the pass of the time.
Closterium by Rogelio Moreno G., on Flickr
Closterium by Rogelio Moreno G., on Flickr
Micrasterias laticeps by Rogelio Moreno G., on Flickr
Micrasterias laticeps by Rogelio Moreno G., on Flickr
Micrasterias furcata by Rogelio Moreno G., on Flickr
Micrasterias radiosa by Rogelio Moreno G., on Flickr
Rogelio
All images were done with the Plan Apo 20x/0.75 and the UV-2B cube with auto fluorescence. You have to do the stack quicly because the specimen auto-fluorescence get dim with the pass of the time.
Closterium by Rogelio Moreno G., on Flickr
Closterium by Rogelio Moreno G., on Flickr
Micrasterias laticeps by Rogelio Moreno G., on Flickr
Micrasterias laticeps by Rogelio Moreno G., on Flickr
Micrasterias furcata by Rogelio Moreno G., on Flickr
Micrasterias radiosa by Rogelio Moreno G., on Flickr
Rogelio
Last edited by RogelioMoreno on Tue Sep 24, 2013 4:24 am, edited 1 time in total.
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Beautiful results. Now you need to figure out which antifade reagents are the least damaging to algae. You might try some dilute p-Phenylenediamine or DABCO (start with perhaps <= 20 micromolar in the water you use to mount the specimens and work up to larger concentrations if required) to try and reduce the photobleaching. Deconvolution results look great, can you please include some details as to which algorithm, how many slices, which model parameters etc that you are using?
David
David
I'm sure we will see one of them in top 10 of small world or bioscapes (: great!
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I'm looking for the the extemely rare V-IM magnification changer for the E800 scope. If you have seen a listing or have one for sale please let me know.
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Thank you for your comments.
Ernst, I am getting my samples from lake Miraflores (this lake has some degree of salt because it is a reservoir for the Panama canal). If the specimen on picture #4 has double tips on the side lobes then I am finding single and double.
Ecki, I am using the 3o days trial of cellSens and AutoQuant X3.
David thank you for the tips about the p-Phenylenediamine, do you know where I can get it?
I am using the default settings on both cellSens and AutoQuant 3D deconvolution, no SA detection.
#1: 2322 x 1179 x 24 slides
#2: 2498 x 1339 x 14 slides
#3: 1851 x 1750 x 20 slides
#4: 1684 x 1688 x 23 slides
#5: 2061 x 2010 x 21 slides
#6: 2161 x 2073 x 16 slides
All with the Plan Apo 20x/0.75 and 1 um step.
Rogelio
Ernst, I am getting my samples from lake Miraflores (this lake has some degree of salt because it is a reservoir for the Panama canal). If the specimen on picture #4 has double tips on the side lobes then I am finding single and double.
Ecki, I am using the 3o days trial of cellSens and AutoQuant X3.
David thank you for the tips about the p-Phenylenediamine, do you know where I can get it?
I am using the default settings on both cellSens and AutoQuant 3D deconvolution, no SA detection.
#1: 2322 x 1179 x 24 slides
#2: 2498 x 1339 x 14 slides
#3: 1851 x 1750 x 20 slides
#4: 1684 x 1688 x 23 slides
#5: 2061 x 2010 x 21 slides
#6: 2161 x 2073 x 16 slides
All with the Plan Apo 20x/0.75 and 1 um step.
Rogelio