Ixodes - Polarized light + stacking + panorama

[Fredlab]

Hello

I have not had much time this summer to post some of my works. I spent my time doing panoramas on thin sections of rocks (I will show it in other threads).
I took the opportunity my microscope is in "polarized/analized light mode" to see what gave few biologic slides.
A tick, prepared by a friend, gave a spectacular result.



Striated muscles appear clearly.
In larger size - here

About technique used : 12 files of 50 images stacked (SPlan 10 + NFK 2,5 + EOS 550D), stitched with AutoPano.
The original size of this panorama is 16000 pixels length.

[Fredlab]

In comparaison with other technics.

Single raw shots


(achromatic condenser 0,9 - SPlan 10x - brightfield illumination)


(brightfield oblique illumination)


(polarized non analized light)


(polarized analized light)


(SPlan 20x)


(20x + stack of 25 picts - in real size - HERE in comparaison with one shot of the file - HERE)


(SPlan 10x + aplanetic condenser 1,4 + DIC)


(DIC + SPlanApo 40x)

[Cactusdave]

Excellent pictures. I especially like the first one. Your friend has done a good job of the preparation and mounting.

[Fredlab]

Excellent pictures. I especially like the first one. Your friend has done a good job of the preparation and mounting.

Many thanks

Last work



panorama of 6 pictures, taken with one SPlan 4.

[Cactusdave]

I am very impressed with this series. Ticks are often very dark and with quite a hard carapace, making them difficult to mount. The usual methods to clear and soften them involves alkali which dissolves muscles, leaving a less interesting specimen.
Perhaps your friend was lucky to find a tick which did not need this harsh treatment, but I would be interested to know his method of preparing this specimen if he was prepared to share it.

[Fredlab]

Thanks again for your interest.

I am forwarding your request to my friend.

[RogelioMoreno]

Very nice set!, specially #1.

Rogelio

[Jacek]

well done, very nice

[JH]

The first one is very good!
Jörgen