Gloeotrichia natans

Ecki · Post by **Ecki** » Sun Sep 08, 2013 11:33 am

While walking around Berlin's Botanical Garden I noticed some small yellow brownish mucilage balls in a pond. Under the microscope these balls appeared to be colonies of the Cyanobacteria Gloeotrichia natans. The basal ball is the heterocyst. The trichome has a distinct curved form.

40x, Jamin-Lebedeff Interference

63x, DIC

63x, Autofluorescence

The first two images were shot with an old shiny black Zeiss Standard WL, equipped with the extinct Jamin-Lebedeff interference contrast., that I managed to acquire recently.

Best regards,
Ecki

Jacek · Post by **Jacek** » Sun Sep 08, 2013 11:49 am

The first two photos of what the technology / contrast /?
interesting

Ecki · Post by **Ecki** » Sun Sep 08, 2013 12:09 pm

http://microscope.database.free.fr/Acce ... bedeff.pdf

This describes the interference method best.

RogelioMoreno · Post by **RogelioMoreno** » Mon Sep 09, 2013 8:41 am

Ecki,

Very nice and intesresting specimen and Jamin-Lebedeff Interference
method!

Rogelio

carlos.uruguay · Post by **carlos.uruguay** » Mon Sep 09, 2013 8:25 pm

Impressive!!

curt0909 · Post by **curt0909** » Tue Sep 10, 2013 1:10 am

Great! I was waiting for these since the last post when you mentioned you had the scope. The results are better than I expected. I'm looking forward to seeing more of these.

Pau · Post by **Pau** » Wed Sep 11, 2013 10:22 am

Very nice images and super interesting Jamin-Lebedeff Interference method, maybe old but undoubtely new for most of us.

The J-L images are excellent but do show less resolution than DIC, of course it isn't surprising as 40X (dry?) vs 63X (1.4?) may not a be a fair comparison. Did you compare the J-L images with DIC 40X ones with objectives of similar NA?