Playing with fluorescence illumination (images added)

Images made through a microscope. All subject types.

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RogelioMoreno
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Playing with fluorescence illumination (images added)

Post by RogelioMoreno »

Here are my first test of my fluorescence illumination system.
All with UV-2B cube and polarized light (composite), stacked, Plan Apo 20x/0.75, Canon T3i mounted on the front port of the TE300.

Image
Micrasterias radiosa by Rogelio Moreno G., on Flickr


Image
Micrasterias rotata by Rogelio Moreno G., on Flickr

Hope you enjoy them.

Rogelio
Last edited by RogelioMoreno on Tue Aug 27, 2013 3:55 pm, edited 1 time in total.

Jacek
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Post by Jacek »

Congratulations, secondary beautiful picture

Litonotus
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Post by Litonotus »

second one is great (: fluorescence becomes popular here (:
my FB page

I'm looking for the the extemely rare V-IM magnification changer for the E800 scope. If you have seen a listing or have one for sale please let me know.

Hindpool
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Post by Hindpool »

The detail is superb and especially so in the second photo. Wonderful work Rogelio - and to read that you are just starting with Fluorescence makes me wonder just how much more is to come! Congratulations.

sebba28
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Post by sebba28 »

Exellent shots, Rogelio
I can"t wait to see more your fluorescence illumination pictures.

discomorphella
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Post by discomorphella »

Hi Rogelio,

Superb results. I hope you enjoy epifluorescence as much as I do. Next we'll get you and Waldo using fluorescent staining as well....
What were the individual exposure times?

David

g4lab
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Post by g4lab »

shameless but non commercial sales promotion
Anybody needing a fluorescent light source should send me a private message. I have HBO arc lamp outfits available and also the Lumen Dynamics X-Cite metal halide which Rogelio is using. end promotion
Beautiful job , as always, Rogelio!

RogelioMoreno
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Post by RogelioMoreno »

Thank you very much for your kind words.

David, I will enjoy it. Are the fluorescent staining safe?
The fluorescence were 3.2 seconds with the X-Cite's diaphragm set to 12% (to avoid photo-bleach, I have to try with greater aperture), the polarized with flash 1/200.

Gene, thank you for the deal of the X-Cite illuminator.

Rogelio

René
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Post by René »

Wow, these are fantastic! Thansk for showing!

René

luchalagos
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Post by luchalagos »

beautiful Rogelio!! congrats!!!

pwnell
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Post by pwnell »

Hey Rogelio,

These are really great. Seems like you managed very well to prevent the fluorescence from the chloroplast leaking - these are sharp.

Ecki
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Post by Ecki »

Very nice!

RogelioMoreno
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Post by RogelioMoreno »

Thank you again for your comments.

Anybody interested in knowing how to overlay fluorescence and brightfield (also can be polarized, DIC, phase contrast, etc) check the following tutorial:

http://www.youtube.com/watch?v=WWQhMnC3BKU

Rogelio

RogelioMoreno
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Post by RogelioMoreno »

Here a few more.

Image
Closterium by Rogelio Moreno G., on Flickr


Image
Micrasterias furcata by Rogelio Moreno G., on Flickr

Image
Spirogyra by Rogelio Moreno G., on Flickr


Rogelio

Litonotus
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Post by Litonotus »

abslolutely brilliant and visually stunning! (:
my FB page

I'm looking for the the extemely rare V-IM magnification changer for the E800 scope. If you have seen a listing or have one for sale please let me know.

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