Plants & Algae

Images made through a microscope. All subject types.

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pwnell
Posts: 2029
Joined: Fri Dec 18, 2009 4:59 pm
Location: Tsawwassen, Canada

Plants & Algae

Post by pwnell »

Image
20130810-DSLR_IMG_1017-Edit.jpg by pwnell, on Flickr
Leaf, 2x, EPI-XC-POL, HF B

Image
20130810-DSLR_IMG_1113-Edit.jpg by pwnell, on Flickr
Pine needles, 2x, FLUO-C4, cellSens deconvolve spherical

Image
20130810-DSLR_IMG_1116-Edit.jpg by pwnell, on Flickr
Pine needles, 40x/0.6, EPI-XC-POL, HF C

Image
20130812-DSLR_IMG_0024.jpg by pwnell, on Flickr
Algae from minnekhada park, 20x, FLUO-C4

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20130812-DSLR_IMG_0030.jpg by pwnell, on Flickr
Algae from minnekhada park, 40x, DIC

Image
20130812-DSLR_IMG_0032.jpg by pwnell, on Flickr
Algae from minnekhada park, 40x, DIC

Image
20130812-DSLR_IMG_0035.jpg by pwnell, on Flickr
Algae from minnekhada park, 40x, FLUO-C4

Image
20130812-DSLR_IMG_0037.jpg by pwnell, on Flickr
Algae from minnekhada park, 40x, POL

Image
20130812-DSLR_IMG_0081-Edit.jpg by pwnell, on Flickr
Algae from minnekhada park, 40x,DIC, HF B (8,8)

emsiem
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Location: Zagreb, Croatia
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Post by emsiem »

Beautiful!
Nenad

RogelioMoreno
Posts: 2962
Joined: Fri Nov 20, 2009 11:24 am
Location: Panama

Post by RogelioMoreno »

Beautiful set!

I would like to see the second one without deconvolution (to compare).

Rogelio

pwnell
Posts: 2029
Joined: Fri Dec 18, 2009 4:59 pm
Location: Tsawwassen, Canada

Post by pwnell »

Thanks. Here (the difference is more striking at 100%):

Image

Jacek
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Joined: Sun Oct 02, 2011 7:00 am
Location: Poland

Post by Jacek »

Beautiful as always

discomorphella
Posts: 606
Joined: Sun Oct 01, 2006 7:26 pm
Location: NW USA

Post by discomorphella »

Excellent autofluorescence shots. One thing I really like about having an aberration model in the deconvolution to take out the spherical terms is that you don't have to really fuss with the correction collar...of course you have to take a large stack of images, nature doesn't give anything away for free...next you'll have to try mixing in some Calcofluor to stain the cell walls into your pond slide (and then, WITH PROPER GLOVES and safety goggles etc, some TOTO or DAPI to light up the nuclei).

David

pwnell
Posts: 2029
Joined: Fri Dec 18, 2009 4:59 pm
Location: Tsawwassen, Canada

Post by pwnell »

Thanks. My problem is that I cannot get any of those stains shipped to me as they will only be sold to educational institutions.

jswatts
Posts: 87
Joined: Mon Apr 08, 2013 11:34 pm
Location: Brisbane, Australia
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Post by jswatts »

Yep, excellent images. FYI, calcofluor is a paper whitener, so you might be able to find another source for it.

Cactusdave
Posts: 1631
Joined: Tue Jun 09, 2009 12:40 pm
Location: Bromley, Kent, UK

Post by Cactusdave »

Ebay item 350101958297 is calcofluor white. The seller also has other fluorescent stains available.
Leitz Ortholux 1, Zeiss standard, Nikon Diaphot inverted, Canon photographic gear

carlos.uruguay
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Post by carlos.uruguay »

Very beautiful

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