Amoeba proteus

Images made through a microscope. All subject types.

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Olympusman
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Joined: Sun Jan 15, 2012 12:31 pm

Amoeba proteus

Post by Olympusman »

Image

Image
Michael Reese Much FRMS EMS Bethlehem, Pennsylvania, USA

Cyclops
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Post by Cyclops »

Absolutely stunning! I still can never find an amoeba!
Canon 5D and 30D | Canon IXUS 265HS | Cosina 100mm f3.5 macro | EF 75-300 f4.5-5.6 USM III | EF 50 f1.8 II | Slik 88 tripod | Apex Practicioner monocular microscope

Olympusman
Posts: 5133
Joined: Sun Jan 15, 2012 12:31 pm

Finding amoeba

Post by Olympusman »

Amoeba appear to be very environment sensitive. At this pond I took a dip from the bottom and a dip from the top. I found no amoebae in the bottom dip but the top dip is very rich in proteus and difflugia (both oblonga and globulosa.
When I do get a dip rich in amoebae I tend to concentrate on the amoebae until the sample goes stale.
Yesterday I tried some vital staining using Methylene Blue, Eosin, Fuchsin, Carmine, Iodine Tincture, Haematoxylin, Amaranth, Crystal Violet and Wright's Blood Stain and found the proteus was impervious to all the stains, though I saw a slight tinge in the nuclei with Haematoxylin.
Looking at the light image above, the small bumps on the tail end of the amoeba suggest this may not be an Amoeba proteus but rather a Polychaos dubium. Aslo a protues flows through one pseudopod at once whereas a dubium flows through several simultaneously.
Michael Reese Much FRMS EMS Bethlehem, Pennsylvania, USA

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