Fern stomata

Images made through a microscope. All subject types.

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pwnell
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Fern stomata

Post by pwnell »

There was a discussion surrounding the black "bars" in the fluorescence shot of these fern stomata before. This may be a better photo showcasing the interesting pattern.

Image
20130810-DSLR_IMG_0837.jpg by pwnell, on Flickr
Fern stomata, 40x/0.6,EPI-XC-POL, HF B (15,3)

Image
20130810-DSLR_IMG_0866.jpg by pwnell, on Flickr
Fern stomata, 40x/0.6,FLUO-C4, HF B (40,2)

RogelioMoreno
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Post by RogelioMoreno »

Nice, specially the last one.

Rogelio

Cyclops
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Post by Cyclops »

Very interesting!
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Pau
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Post by Pau »

Nice images as usual, Waldo. I like the comparison with the same field.
Pau

Jacek
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Post by Jacek »

beautiful

Fredlab
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Post by Fredlab »

Excellent shot
As always.

Fluo is amazing.
You must try with only epidermis (chlorophyl in stomatic cell only)
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pwnell
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Post by pwnell »

Thanks. What do you mean by chlorophyll in stomatic cell only? Do you mean a more magnified photo?

Fredlab
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Post by Fredlab »

Epidermic cells are not chlorophyllian, only stomatic cells contain chloroplasts.
I think, epidermis (easy to collect) with stomates, in fluorescence, will be nice.
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pwnell
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Post by pwnell »

Where would I collect these kind of subjects? I thought chloroplasts fill the epidermis of all plant leaves?

Cyclops
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Post by Cyclops »

pwnell wrote: I thought chloroplasts fill the epidermis of all plant leaves?
So did I, considering chlorophyll is what makes plants green! Plus in most plants the stomata are only found on the underside to reduce transpiration in hot weather. If only stomatic cells had chlorophyll then most plants would appear very pale!
Canon 30D | Canon IXUS 265HS | Cosina 100mm f3.5 macro | EF 75-300 f4.5-5.6 USM III | EF 50 f1.8 II | Slik 88 tripod | Apex Practicioner monocular microscope

Fredlab
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Post by Fredlab »

pwnell wrote:Where would I collect these kind of subjects? I thought chloroplasts fill the epidermis of all plant leaves?
Simply tear sheet obliquely. A white "skin" will appear at the torn area ... with fine forceps, you removed a fragment that can be even greater (bottom panel - it comes off easily)

Image
(panoramic shot - bigger size - HERE)
I apologise for my poor english
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