Hydra in darkfield

Images made through a microscope. All subject types.

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Olympusman
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Joined: Sun Jan 15, 2012 12:31 pm

Hydra in darkfield

Post by Olympusman »

Last week I fabricated some darkfield stops to go into the filter holder on the condenser of my main trinocular.

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They are made from washers and the hole in the center is filled with the plugs from a paper punch. from left to right they are 15.6mm, 12.7mm and 9.4mm.
This is a 5x shot of a hydra:

Image

This a 10x shot with an Olympus Dplan 10x 0.25 160/.17 objective:

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This is a 20x shot of the hydra's tentacles taken with a generic 20x 0.40 160/.17 employing a false DIC technique. The darkstop in the filter holder is swung sideways from the center position under the condenser so that half of the condenser is darkened and half is passing unretarded light.

Image
Michael Reese Much FRMS EMS Bethlehem, Pennsylvania, USA

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