Simocehalus vetulus NCG imges

Litonotus · Post by **Litonotus** » Sun Jun 09, 2013 10:09 am

no-cover-glass images

20x images, it is good that I have not sold the PF objective yet (: there would be no use of the PA in this case...

Charles Krebs · Post by **Charles Krebs** » Sun Jun 09, 2013 2:17 pm

Nice shots. Shooting without cover-slips can create some optical difficulties, but is sure makes for some interesting backgrounds with your DIC!

Litonotus · Post by **Litonotus** » Sun Jun 09, 2013 9:04 pm

thank you (: playing with decentering DIC elements can create really interesting effects, very colourful and close to polarized light microscopy (:

anything over NA 0,3-0,4 doesn't look good... low class objectives are better for that (;

curt0909 · Post by **curt0909** » Sun Jun 09, 2013 9:16 pm

Sharpness is pretty good here without cover slip. I'd assume if you used a Plan Apo with this method it would quickly become a water immersion technique.

Litonotus · Post by **Litonotus** » Sun Jun 09, 2013 10:17 pm

thanks (:

curt0909 wrote: I'd assume if you used a Plan Apo with this method it would quickly become a water immersion technique.

indeed (; so I have not tried that at all (;

arturoag75 · Post by **arturoag75** » Mon Jun 10, 2013 12:10 am

very nice!