Quiz...What is it? (We have a correct answer!)

Images made through a microscope. All subject types.

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Charles Krebs
Posts: 5865
Joined: Tue Aug 01, 2006 8:02 pm
Location: Issaquah, WA USA

Post by Charles Krebs »

Thanks all for the comments!
(... and I think we need to give Rik a 24 hour handicap when it comes to guessing these things...) :wink:

I still do not see whole picture how did you approach this setup with microscope objective...
I've set up a Nikon Measuring Microscope to work with subjects that need to be illuminated from the top in some manner. My normal biological microscopes felt too confining with insufficient room to set up my preferred lighting arrangements. I use long working distance objectives (either "M-type" designed to be used with no cover-slip, or ones with a low enough NA that a cover-slip doesn't matter). This arrangement provides plenty of room to illuminate from above (and/or from below if desired), and is extremely versatile. The basic MM-11 set-up can be seen here:
http://www.photomacrography.net/forum/v ... hp?t=17656

However since that post I have replaced the tubes and optics between the stand and the camera with an Olympus super-wide trinocular viewing head (model U-SWTR-3). This head provides an 80/20 light split so I can observe through the eyepieces and photograph simultaneously. (I should update that older post with the way it currently is being used).

The last, older picture above that shows the entire pupa was made using the Canon 65mm MPE lens with the pupa in a tiny "tank" made from microscope slide cover glass.

Here's something that might be useful to a few others in the group...

One lighting "component" I sometimes use (and did use for these shots) is a way of providing the colored background. It can be tricky to position a flash or continuous light under the stage I use. Mirrors can work well so I got one of those "Spy Scope, Right Angle Mirrors" off Ebay. Works great. I can direct a flash or continuous light directly into the side port. Over the top I can place a diffuser, colored filter/gel, or in this case simply a blue piece of paper. Here's a shot of the bottom "background light" that provided the background color for the above shots. In this instance even though the paper on top is a light blue color, you can vary the background from very deep blue to nearly white depending on the intensity of the flash relative to the main subject light above.


Walter Piorkowski
Posts: 693
Joined: Mon Aug 14, 2006 6:42 pm
Location: South Beloit, Ill

Post by Walter Piorkowski »

Remarkable work Charles. Patiance, perseverence and skill.

Posts: 301
Joined: Wed Nov 18, 2009 2:41 pm
Location: Netherlands

Post by Ferry »

I don't know what is more astonishing, Charles remarkable photography or Riks correct answer! :D

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