Filaments of the Spiderwort Blossom.

Images made through a microscope. All subject types.

Moderators: rjlittlefield, ChrisR, Chris S., Pau

Walter Piorkowski
Posts: 693
Joined: Mon Aug 14, 2006 6:42 pm
Location: South Beloit, Ill

Filaments of the Spiderwort Blossom.

Post by Walter Piorkowski »

Image

Image

Image

Image

Leitz Ortholux microscope
4X Leitz projection eyepiece plus 1/3x relay lens


Image 1- - Leitz 6.3x Plan Apo, 91 images at .001" increments.
Portions of individual filament strands. Magnification on sensor 10X.

Image 2- - Leitz 4X Plan Fluorite, 109 images at .002" increments.
Filament strands and one anther head. Magnification on sensor 7X.

Image 3- - Leitz 2.5X Plan Fluorite, 90 images at .005" increments.
Stigma and anther head, with pollen grains, plus filaments. Magnification on sensor 4X.

Image 4- Not a photomicrograph, but a macro shot at approximately 1:1 to illustrate the full filament structure. This is a different plant of the same species with a blue color. Sony NEX-5N with 30mm macro. 73 images at .5mm increments. Uncut subject in it’s natural state.

Every spring I enjoy looking at the small wild flowers under some magnification. Most of the flowers seem to follow the same patterns of growth, anthers and stigmas protruding up out of the base alone. In looking at the flowers of the tradesantia or spiderwort, I was intrigued by the hairy material under the stigma and anthers. These turned out not to be hairy at all, they were more like a beaded necklace.

Walt


Diffused Fiber Optic illumination
Canon 50D
Zerene PMax and Photoshop processing.

rjlittlefield
Site Admin
Posts: 23363
Joined: Tue Aug 01, 2006 8:34 am
Location: Richland, Washington State, USA
Contact:

Post by rjlittlefield »

Interesting, and very beautiful! I do not recall seeing this string-of-beads structure before.

A couple of questions about the stacking...
1. What are the NA's on those objectives?
2. What sort of stepping rig for a microscope works in increments like 0.001" ?

--Rik

Walter Piorkowski
Posts: 693
Joined: Mon Aug 14, 2006 6:42 pm
Location: South Beloit, Ill

Post by Walter Piorkowski »

Thanks Rik, glad you enjoyed them.
The NA's are as follows 6.3x=0.20, 4x=0.14, 2.5x 0.08 NA. Nothing out of the ordinary.
I am including a photo of the rig that provides for thousanths increments. It is just a dial indicator projecting out from the solid frame that supports the camera. A device I made myself. The microscope is still moved by the stage positioning dials but I read the indicator. It can be placed against the stage at any hieght.

Walt

Imagealt

Franz Neidl
Posts: 747
Joined: Wed Jan 14, 2009 11:59 am
Location: Italy

Post by Franz Neidl »

Hello Walter,
congratulations for your pictures. I like very much your 3. photo.
I too I like Tradescantia - specially the fascinating mouvements in the cells of the filaments.

Franz

ChrisR
Site Admin
Posts: 8661
Joined: Sat Mar 14, 2009 3:58 am
Location: Near London, UK

Post by ChrisR »

As it's Walt, I looked up the name.
Apparently:
The name Spiderwort is attributed to the observation that the monocotyledonous, grass- like leaves of the plant are suggestive of a crouching spider.
Six stamens though - do any have eight? :P

That filament structure is surprising, and very attractive.

Pau
Site Admin
Posts: 5995
Joined: Wed Jan 20, 2010 8:57 am
Location: Valencia, Spain

Post by Pau »

Wow!, excellent series, both in tech and aesthetically aspects.

Very interesting microscope setup. I'm a bit surprised about yout use of a projective eyepiece with a relay lens. Is the 0.3X lens focused to infinite?
Pau

JH
Posts: 1305
Joined: Sat Mar 09, 2013 9:46 am
Location: Vallentuna, Stockholm, Sweden
Contact:

Post by JH »

Inspiring, could you say something about your light source and difuser?
Jörgen

Walter Piorkowski
Posts: 693
Joined: Mon Aug 14, 2006 6:42 pm
Location: South Beloit, Ill

Post by Walter Piorkowski »

Hi Franz. Thank you, it is an interesting and beautiful flower.

Thanks a lot Chris! I was hoping to avoid a connection to my work with spiders. No chance of that now! :roll:

Pau. My projection set up was set up many years ago. At that time, there did not seem to be any special position for the relay lens above the projection eyepiece. To be honest I followed closely the original arrangement established by Leitz in their MIKAS micro attachment. I changed out their 10X Periplan eyepiece for the 4x projection unit eyepiece.

Hi Jorgen. My light source for these images was a two probe fiber optic illuminator. The flower blossom was placed inside a white plastic diffuser ring that was slightly higher than the subject. The ring was illuminated at 2 points, 90 degrees from each other.

Walt

RogelioMoreno
Posts: 2975
Joined: Fri Nov 20, 2009 11:24 am
Location: Panama

Post by RogelioMoreno »

Walt,

Very nice, specially #1.

Rogelio

Walter Piorkowski
Posts: 693
Joined: Mon Aug 14, 2006 6:42 pm
Location: South Beloit, Ill

Post by Walter Piorkowski »

Thank you Rogelio. I enjoy your remarkable images.
Walt

arturoag75
Posts: 1600
Joined: Fri Feb 05, 2010 1:05 am
Location: italy
Contact:

Post by arturoag75 »

Amazing shots :shock:
arturo

Jean-marc
Posts: 283
Joined: Sat Oct 06, 2007 2:57 pm
Location: France
Contact:

Post by Jean-marc »

Hello,

Your pics are really beautiful, looks like some SEM pics with artificial color traitment.

JM

Walter Piorkowski
Posts: 693
Joined: Mon Aug 14, 2006 6:42 pm
Location: South Beloit, Ill

Post by Walter Piorkowski »

Arturo and Jean. Thank you for kind comments.
Walt

Charles Krebs
Posts: 5865
Joined: Tue Aug 01, 2006 8:02 pm
Location: Issaquah, WA USA
Contact:

Post by Charles Krebs »

Really gorgeous shots Walt!
Beautiful subject!

descall
Posts: 178
Joined: Sat Oct 01, 2011 3:12 pm
Location: United Kingdom

Post by descall »

Deeply and simply beautiful. Best regards, Des

Post Reply Previous topicNext topic