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Yes, it is primarily the very short duration of the electronic flash. The Vivitar 283 I use is about 1/1000 second at full power. With the 60X objective I am at about "-3" (three stops under full power). While I have no way of measuring it, this is likely somewhere between 1/4000 and 1/8000 second. When I use flash for shots like this I don't work from live-view. Typically the camera body is set to use mirror lock-up as well. Then, as I am getting ready to take a shot I hit the release one time to lock-up the mirror. When I have the subject in the frame properly and in focus, I release the shutter to take the picture. (This avoids any mirror "slap" and vibration. Even though the flash is of very short duration, I want to avoid the vibration from the mirror).But I dont still understand why the pictures are so clear (for exemple the cilia). It seems that there is not a minimum of vibration in the camera.
Is the secret the very short duration of the flash? Did you use your adapted Vivitar 283 flash for these 3 pictures with a very short flash time?