Micrasterias thomasiana, divisions and some special views

myriophyllum · Post by **myriophyllum** » Tue May 21, 2013 1:27 pm

Hi,

some times ago I showed a video about the division of Euastrum oblongum http://www.youtube.com/watch?v=gzh5j3NJ5-Q.
Now the second part of my desmid divisions: A film of Micrasterias thomasiana var. notata. Some scenes have been recorded in time lapse (up to 125x).

You can watch the video here:

http://www.youtube.com/watch?v=0P7RQV51XvU

Some stills out of that video:

Bacteria on the string between two cells

The intercellular connections have in one scene (around 0:35) been pointed out by putting some chinese ink into the water.

An empty shell

And some moments of a division, it took about 4 hours from the first to the last image:

Illumination: Darkfield, phase contrast and asymmetric Rheinberg/Col mixture
Zeiss Standard 18; Planapos 4, 10, 25, 40 and 63 Ph; Ultrakondensor; Achr.-Apl. 1.4;
Kpl 20x/8; S-Kpl 16x/12,5; S-Kpl 10x/20.

I hope you enjoy it.

Greetings

Jens

pwnell · Post by **pwnell** » Tue May 21, 2013 2:01 pm

These are great and very informative, thanks.

Pau · Post by **Pau** » Tue May 21, 2013 2:08 pm

Excellent!!!

curt0909 · Post by **curt0909** » Tue May 21, 2013 2:45 pm

Wow! Super job. I really appreciate the effort and time that went into making this. You're a master of Rhienberg oblique illumination.

RogelioMoreno · Post by **RogelioMoreno** » Tue May 21, 2013 4:06 pm

Very informative.

Rogelio

carlos.uruguay · Post by **carlos.uruguay** » Tue May 21, 2013 8:55 pm

Marvelous!!
I am grateful to you that you share videos and photos of this excellent work
carlos

Jacek · Post by **Jacek** » Tue May 21, 2013 9:38 pm

myriophyllum · Post by **myriophyllum** » Thu May 23, 2013 7:41 am

Hi,

many thanks for all Your comments,
I enjoyed them a lot. :-))

Greetings

Jens

harisA · Post by **harisA** » Thu May 23, 2013 1:02 pm

Very beautiful images.

I would like to know how you take photos with the zeiss standard. I suspect that you have the trinocular head and you use a s-klp eyepiece.What additional lens are you using in order to project the image on camera sensor.
Also what camera do you have?

Thank you in advance

Haris.A

myriophyllum · Post by **myriophyllum** » Thu May 23, 2013 2:45 pm

Hi Haris,

thank You.
It's right, I use trinocular head and Kpl or S-Kpl eyepieces.
Then the image is projected by a Zeiss T2 adapter (0.25x) with 63 mm
lens inside onto the chip of a Canon EOS 5DII.
That works very fine for filming. :-)

Best wishes

Jens

harisA wrote:Very beautiful images.

I would like to know how you take photos with the zeiss standard. I suspect that you have the trinocular head and you use a s-klp eyepiece.What additional lens are you using in order to project the image on camera sensor.
Also what camera do you have?

Thank you in advance

Haris.A

Wim van Egmond · Post by **Wim van Egmond** » Fri May 24, 2013 12:10 am

Very nice results. Since we usually observe microorganisms under a coverslip we often don't see how the mucilage forms a stalk. I have a petridish with M. thomasiana alive for more than half a year.
When you put them under the stereomicroscope you can see that it is almost like a forest of palm trees.

Wim

harisA · Post by **harisA** » Sat May 25, 2013 6:21 am

Thank you Jens for the info.

One more thing.Is there significant difference in image quality between s-kpl and kpl eyepieces ?

Thank you in advance

Haris.A

myriophyllum · Post by **myriophyllum** » Sun May 26, 2013 10:54 am

Hi Harris,

i have owned both, the Kpl-W 10x/20 Br foc. and the S-Kpl 10x/20 Br.
With the S-Kpl You have less monochromatic image artifacts like
coma and astigmatism in the outer areas of the image.
So if You can use large fields the S-Kpl is the better choice.
I sold my pair of Kpl-W 10x/20...

Greetings

Jens

harisA wrote:Thank you Jens for the info.

One more thing.Is there significant difference in image quality between s-kpl and kpl eyepieces ?

Thank you in advance

Haris.A