Micrasterias thomasiana, divisions and some special views

Images made through a microscope. All subject types.

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myriophyllum
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Location: Schaumburg, north of Germany
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Micrasterias thomasiana, divisions and some special views

Post by myriophyllum »

Hi,

some times ago I showed a video about the division of Euastrum oblongum http://www.youtube.com/watch?v=gzh5j3NJ5-Q.
Now the second part of my desmid divisions: A film of Micrasterias thomasiana var. notata. Some scenes have been recorded in time lapse (up to 125x).

You can watch the video here:

http://www.youtube.com/watch?v=0P7RQV51XvU



Some stills out of that video:

Bacteria on the string between two cells

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The intercellular connections have in one scene (around 0:35) been pointed out by putting some chinese ink into the water.

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An empty shell

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And some moments of a division, it took about 4 hours from the first to the last image:

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Illumination: Darkfield, phase contrast and asymmetric Rheinberg/Col mixture
Zeiss Standard 18; Planapos 4, 10, 25, 40 and 63 Ph; Ultrakondensor; Achr.-Apl. 1.4;
Kpl 20x/8; S-Kpl 16x/12,5; S-Kpl 10x/20.

I hope you enjoy it.

Greetings

Jens

pwnell
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Location: Tsawwassen, Canada

Post by pwnell »

These are great and very informative, thanks.

Pau
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Post by Pau »

Excellent!!!
Pau

curt0909
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Post by curt0909 »

Wow! Super job. I really appreciate the effort and time that went into making this. You're a master of Rhienberg oblique illumination.

RogelioMoreno
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Post by RogelioMoreno »

Very informative.

Rogelio

carlos.uruguay
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Post by carlos.uruguay »

Marvelous!!
I am grateful to you that you share videos and photos of this excellent work
carlos

Jacek
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Post by Jacek »

:shock:

myriophyllum
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Post by myriophyllum »

Hi,

many thanks for all Your comments,
I enjoyed them a lot. :-))

Greetings

Jens

harisA
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Post by harisA »

Very beautiful images.

I would like to know how you take photos with the zeiss standard. I suspect that you have the trinocular head and you use a s-klp eyepiece.What additional lens are you using in order to project the image on camera sensor.
Also what camera do you have?

Thank you in advance

Haris.A

myriophyllum
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Location: Schaumburg, north of Germany
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Post by myriophyllum »

Hi Haris,

thank You.
It's right, I use trinocular head and Kpl or S-Kpl eyepieces.
Then the image is projected by a Zeiss T2 adapter (0.25x) with 63 mm
lens inside onto the chip of a Canon EOS 5DII.
That works very fine for filming. :-)

Best wishes

Jens

harisA wrote:Very beautiful images.

I would like to know how you take photos with the zeiss standard. I suspect that you have the trinocular head and you use a s-klp eyepiece.What additional lens are you using in order to project the image on camera sensor.
Also what camera do you have?

Thank you in advance

Haris.A

Wim van Egmond
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Post by Wim van Egmond »

Very nice results. Since we usually observe microorganisms under a coverslip we often don't see how the mucilage forms a stalk. I have a petridish with M. thomasiana alive for more than half a year.
When you put them under the stereomicroscope you can see that it is almost like a forest of palm trees.

Wim

harisA
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Joined: Sun Jul 03, 2011 9:31 am
Location: Greece

Post by harisA »

Thank you Jens for the info.

One more thing.Is there significant difference in image quality between s-kpl and kpl eyepieces ?

Thank you in advance

Haris.A

myriophyllum
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Location: Schaumburg, north of Germany
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Post by myriophyllum »

Hi Harris,

i have owned both, the Kpl-W 10x/20 Br foc. and the S-Kpl 10x/20 Br.
With the S-Kpl You have less monochromatic image artifacts like
coma and astigmatism in the outer areas of the image.
So if You can use large fields the S-Kpl is the better choice.
I sold my pair of Kpl-W 10x/20...

Greetings

Jens
harisA wrote:Thank you Jens for the info.

One more thing.Is there significant difference in image quality between s-kpl and kpl eyepieces ?

Thank you in advance

Haris.A

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