Euphyllia polyp

Images made through a microscope. All subject types.

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pwnell
Posts: 2029
Joined: Fri Dec 18, 2009 4:59 pm
Location: Tsawwassen, Canada

Euphyllia polyp

Post by pwnell »

Below is the polyp under various excitation sources displaying different characteristics. The first is my personal favourite.

In the image below the brown / orange part is the polyp body containing photosynthetic zooxanthellae. The green outer shell is the part I believe that contains GFP pigments. The white "smoke" around the outer layer are thousands of nematocysts that fired due to the stress of being manipulated.
Image
10x, POL

Image
10x, DF

In this DIC image the nematocysts can be seen much more clearly, however the details in the polyp are lost.
Image
10x, DIC

This is a composite image of the blue and green/red fluorescent channels. What is most interesting to note here is that no red is visible, one would expect the zooxanthellae to glow bright red due to the chlorophyll. Since this polyp is highly auto fluorescent in the green wavelength, it overpowers the weaker red fluorescence of the chlorophyll.
Image
10x, FLUO-C4 + DAPI

A stack of 46 images showing fired and one unfired nematocyst, as well as a lone zooxanthellae cell.
Image
40x, DIC, HF A

A stack of 55 images showing that phase contrast is still the clearest way to see nematocysts.
Image
40x, PH, HF A

This is an HDR image composed of two exposures, one for GFP and one for the red chlorophyll fluorescence. This is not too far from how the image looked through the eye piece, the red was just slightly more subdued. Red dots are of course zooxanthellae.
Image
40x, FLUO-C4

Single image showing unfired nematocysts upper left, fired nematocyst casings in the middle and their long barbs bottom right. This layer is actually very thick in the z-axis.
Image
60x, DIC

Finally, to put these images in perspective, here is the coral Euphyllia. What you see in the first couple of photos is one single green polyp.
Image
Canon EOS 1Ds Mark III, EF100 F2.8 Macro IS lens.

Same coral, just under fluorescence (blue excitation with yellow barrier filter). The intensity of the GFP fluorescence is striking.
Image
Canon EOS 5D Mark III, EF100 F2.8 Macro IS lens.

Marek Mis
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Joined: Sat Jul 10, 2010 9:56 am
Location: Suwalki, Poland
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Post by Marek Mis »

Beautiful images ! The first one reminds me a part of the universe.

Marek

pwnell
Posts: 2029
Joined: Fri Dec 18, 2009 4:59 pm
Location: Tsawwassen, Canada

Post by pwnell »

Marek Mis wrote:Beautiful images ! The first one reminds me a part of the universe.

Marek
Reminds me of a sun...

RogelioMoreno
Posts: 2975
Joined: Fri Nov 20, 2009 11:24 am
Location: Panama

Post by RogelioMoreno »

Beautiful set!

Rogelio

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