Purple Spotted Swallowtail (Graphium weiskei) image added

Images made through a microscope. All subject types.

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Charles Krebs
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Purple Spotted Swallowtail (Graphium weiskei) image added

Post by Charles Krebs »

This butterfly has some beautiful and unusual aspects to the wings so I did some shots illuminated differently than my usual approach. There are some brightly colored spot sections in the wings, blue, green and purple. Looked at closely these are not the typical pigmented scales or iridescent scales. The color is in the wing membrane itself, somewhat translucent and deeply colored. Where you might normally see "conventional" scales anchored are short hairlike protuberances. (Perhaps these are considered a type of scale..??) It was interesting that on the opposite side of some of these sections were plain white scales. But when light hit these "scale-less" sections, the white scales on the opposite side glowed with an attractive coloration from the light passing through the wing. So I can imagine these butterflies in flight on a sunny day. When the sun hits the white scales, the spots would obviously appear white. When the wing is positioned so that sun hits the colored wing membrane the same white spots would now take on a different color.

The first image is a cross-eyed stereo pair where an attempt was made to illuminate it so that the structure and "hairs" in these spots can be more readily discerned than in a 2D image.


Image

The next two images are the exact same location on the side of the wing that has the white scales, but has a purple "scale-less" membrane of the opposite side of the wing. The left image is reflected light directly on the scales. The right image is with a light shining through the wing. You can clearly see the purple hue taken on by the white scales.


ImageImage


Below is another shot with light shining through an interesting section of the wing, and the white scales have picked up a beautiful range of blue/purple coloration. The last image shows the exact same area on the wing, but this time with the with the reflected light coming from above.


Image

Image
Last edited by Charles Krebs on Fri May 17, 2013 5:57 pm, edited 1 time in total.

canonian
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Post by canonian »

Again wonderful shots, Charles.
Is this a known fact on these species or did you ran into this phenomenon just by reposition the lighting? It's fascinating !

ChrisR
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Post by ChrisR »

Beautiful.
Not a polarising/retarding effect, is it?

leobalbi
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Post by leobalbi »

:shock:

Amazing!!! Pictures and the butterfly.

Best's
Leonardo Balbi

rjlittlefield
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Post by rjlittlefield »

Very nice! I am not familiar with this species and this effect is completely new to me in butterflies.

The stereo works perfectly.

--Rik

Charles Krebs
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Post by Charles Krebs »

Thanks for the comments!
Not a polarising/retarding effect, is it?
No, I used "straight" tungsten illumination (FO illuminator, 3200K). Didn't see any sign of "structural color" either, appeared to me to be the result of pigments. But these are just cursory observations, I really don't know what actually colors the wing membrane.

Fred,
It is very obvious even under modest magnification. I noticed the color "bleed through" when I was fiddling around with the light trying to get a good portrayal of the "hair-like" protrusions which were difficult to see clearly in flat reflected light.

When looking at the arrangement of scales in the eye-spots in various butterflies and moths I always find it amazing how the different scales "know" where to grow. Here's an interesting articles touching upon some of the genetics involved:
http://www.phschool.com/science/science ... spots.html

pwnell
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Post by pwnell »

Really well done. I like the contrast between the two illumination directions in the second set a lot.

MiB
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Post by MiB »

Great photos!!!
Not a polarising/retarding effect, is it?
No, I used "straight" tungsten illumination (FO illuminator, 3200K). Didn't see any sign of "structural color" either, appeared to me to be the result of pigments. But these are just cursory observations, I really don't know what actually colors the wing membrane.
[/quote]

Could you describe how you set the light for these photos? How many lightsources/reflectrors from which direction, and why?

It would be very helpful for us to see photos of your lighting setups on each photo you show us here to better understand and maybe learn how you do it (if this might be possible).

Many thanks and kind regards,
Michael

Marek Mis
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Post by Marek Mis »

Charlie,

Wonderful images !

Marek

seta666
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Post by seta666 »

Very interesting colour behaviour, I really like the stereo

Regards

RogelioMoreno
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Post by RogelioMoreno »

Charlie,

Beautiful and interesting.

Rogelio

Charles Krebs
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Post by Charles Krebs »

Michael
Could you describe how you set the light for these photos? How many lightsources/reflectrors from which direction, and why?

It would be very helpful for us to see photos of your lighting setups on each photo you show us here to better understand and maybe learn how you do it
If the scales are primarily pigmented I will often use a "ring" diffuser such as seen in this post:
http://www.photomacrography.net/forum/v ... hp?t=19587
This is what was used for the "white" surface illuminated scales (second image above), as well as the last image that was just added. I have been using the Ikea LED lights that many of us like so well, but for these a single fiber optic light guide was the light source.

The top stereo image needed to be illuminated differently in order to clearly show the fine hairlike scales. For that one I used the light (FO light guide) at a low grazing angle directly onto the wing. This was a bit too "hard" and gave totally black shadow areas, so a semi-circular reflector was placed on the opposite side and adjusted in size and location until it provided an adequate fill light.

The two trans-illuminated ("backlit") shots above were taken using a microscope illuminator under the stage with no additional top lights.

If the scales are iridescent they often photograph best with a more all-encompassing light so I'll often use a "full" dome as shown in this post:
http://www.photomacrography.net/forum/v ... hp?t=18579
This diffuser can sometimes be too "flat" with non iridescent scales, but can work well with them if you put the light right up against it.

Often I get by with a single light, but this all determined by looking at the subject as the light(s) are positioned and moved around. If a very even, flat light is required I may need use up to three lights outside of the diffusers.

MiB
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Post by MiB »

Charles,

Many thanks for the description and the links.

Seems very simple. Still my images are far from the quality of yours.

I will search for something I could build a dome out of it. One problem might be that I will photograph a whole wing (stitching) so the distances will be higher and because I cannot move the light with the subject/object lighting may change. Maybe It still will work because each position is max. 2x3mm.

Thanks again for your help and your wonderful images which are a great inspiration!

Michael

Charles Krebs
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Post by Charles Krebs »

Michael,
One problem might be that I will photograph a whole wing (stitching) so the distances will be higher and because I cannot move the light with the subject/object lighting may change.
The way the reflector and lights are used as seen in the following post could work well for stitching since only the subject position is changed:

http://www.photomacrography.net/forum/v ... hp?t=19841

MiB
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Post by MiB »

Charles,

Thanks for the new link.
I will try to employ a dome (I hope I will find something appropriate). At the moment I just use a 4-5 cm high roll of laser printer paper, so most light comes from the sides (about 30° down; a little bit less than the black Ikea lamp on the left side of your lighting setup image).

Do you think a paper-cone mounted to the lens would also do the job or is a dome a much better solution anyway?

Thanks and cheers,
Michael

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