More diatoms, desmids and barnacles.

Images made through a microscope. All subject types.

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pwnell
Posts: 2035
Joined: Fri Dec 18, 2009 4:59 pm
Location: Tsawwassen, Canada

More diatoms, desmids and barnacles.

Post by pwnell »

Image
20130505-DSLR_IMG_0212.jpg by pwnell, on Flickr
Stanley park ocean - diatoms on algae, 40x, FLUO-C4, HF C

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20130505-DSLR_IMG_0227.jpg by pwnell, on Flickr
Stanley park ocean - barnacle, 10x, FLUO-C4

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20130505-DSLR_IMG_0228.jpg by pwnell, on Flickr
Stanley park ocean - barnacle, 10x, FLUO-C4

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20130508-DSLR_IMG_0124.jpg by pwnell, on Flickr
GTF water sample - Euastrum desmid,40x, DIC+QWP, HF B

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20130508-DSLR_IMG_0261.jpg by pwnell, on Flickr
GTF water sample - diatom, 40x, DIC, HF B

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20130508-DSLR_IMG_0274.jpg by pwnell, on Flickr
GTF water sample - diatom, 40x, DIC+QWP, ZS DMap

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20130508-DSLR_IMG_0396.jpg by pwnell, on Flickr
GTF water sample - Euastrum desmid, 60x, QWP+LP, HF B

Jacek
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Post by Jacek »

great set of photos

Marek Mis
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Post by Marek Mis »

Wonderful images !
All of them are great, but my favourite is the third one.

Marek

arturoag75
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Post by arturoag75 »

very nice :wink:

Charles Krebs
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Post by Charles Krebs »

Wonderful shots. The barnacle images are particularly dramatic.

discomorphella
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Location: NW USA

Post by discomorphella »

I really like the fluorescence shots. The barnacles are spectacular. I am assuming that you're not actually using DAPI or other fluorochromes but rather are referring to the labels on your cubes (the dye(s) your cubes were intended for) and these are autofluorescence images.

David

pwnell
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Joined: Fri Dec 18, 2009 4:59 pm
Location: Tsawwassen, Canada

Post by pwnell »

discomorphella wrote:I really like the fluorescence shots. The barnacles are spectacular. I am assuming that you're not actually using DAPI or other fluorochromes but rather are referring to the labels on your cubes (the dye(s) your cubes were intended for) and these are autofluorescence images.

David
Correct - just refers to the cube's excitation/emission spectrum. Everything I do is auto fluorescence.

Thanks for the comments.

Rikisub
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Post by Rikisub »

Amazing!

discomorphella
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Location: NW USA

Post by discomorphella »

Autofluorescence is great. Its amazing how well you can delineate all kinds of structures without any dye at all, and you have surely set the bar for those images, these are just great. For those specimens that are not so inclined, one thing you could try for less "glowy" subjects is Eosin Y or Phloxine B or similar dyes (all chemical relatives of Fluorescein). The fluorescence yield is good, and they are nontoxic (as compared to nasty DNA intercalators DAPI or Propidium, not for the average home user) and have a nice range of colors depending on what they're bound to. They are also comparatively easy to obtain and use (a 0.1% solution in H2O or 70% Ethanol or similar). You can also try Calcofluor, which stains cellulose and chitin. Its just going to be a bit harder to obtain (your DAPI cube will work well for the Calcofluor, and the FITC cube for the Eosin, as you would expect).

David

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