More diatoms, desmids and barnacles.

pwnell · Post by **pwnell** » Tue May 14, 2013 2:20 pm

20130505-DSLR_IMG_0212.jpg by pwnell, on Flickr
Stanley park ocean - diatoms on algae, 40x, FLUO-C4, HF C

20130505-DSLR_IMG_0227.jpg by pwnell, on Flickr
Stanley park ocean - barnacle, 10x, FLUO-C4

20130505-DSLR_IMG_0228.jpg by pwnell, on Flickr
Stanley park ocean - barnacle, 10x, FLUO-C4

20130508-DSLR_IMG_0124.jpg by pwnell, on Flickr
GTF water sample - Euastrum desmid,40x, DIC+QWP, HF B

20130508-DSLR_IMG_0261.jpg by pwnell, on Flickr
GTF water sample - diatom, 40x, DIC, HF B

20130508-DSLR_IMG_0274.jpg by pwnell, on Flickr
GTF water sample - diatom, 40x, DIC+QWP, ZS DMap

20130508-DSLR_IMG_0396.jpg by pwnell, on Flickr
GTF water sample - Euastrum desmid, 60x, QWP+LP, HF B

Jacek · Post by **Jacek** » Tue May 14, 2013 9:40 pm

great set of photos

Marek Mis · Post by **Marek Mis** » Tue May 14, 2013 11:19 pm

Wonderful images !
All of them are great, but my favourite is the third one.

Marek

arturoag75 · Post by **arturoag75** » Tue May 14, 2013 11:22 pm

very nice

Charles Krebs · Post by **Charles Krebs** » Tue May 14, 2013 11:58 pm

Wonderful shots. The barnacle images are particularly dramatic.

discomorphella · Post by **discomorphella** » Wed May 15, 2013 12:24 am

I really like the fluorescence shots. The barnacles are spectacular. I am assuming that you're not actually using DAPI or other fluorochromes but rather are referring to the labels on your cubes (the dye(s) your cubes were intended for) and these are autofluorescence images.

David

pwnell · Post by **pwnell** » Wed May 15, 2013 10:53 am

discomorphella wrote:I really like the fluorescence shots. The barnacles are spectacular. I am assuming that you're not actually using DAPI or other fluorochromes but rather are referring to the labels on your cubes (the dye(s) your cubes were intended for) and these are autofluorescence images.

David

Correct - just refers to the cube's excitation/emission spectrum. Everything I do is auto fluorescence.

Thanks for the comments.

Rikisub · Post by **Rikisub** » Wed May 15, 2013 12:25 pm

Amazing!

discomorphella · Post by **discomorphella** » Wed May 15, 2013 12:46 pm

Autofluorescence is great. Its amazing how well you can delineate all kinds of structures without any dye at all, and you have surely set the bar for those images, these are just great. For those specimens that are not so inclined, one thing you could try for less "glowy" subjects is Eosin Y or Phloxine B or similar dyes (all chemical relatives of Fluorescein). The fluorescence yield is good, and they are nontoxic (as compared to nasty DNA intercalators DAPI or Propidium, not for the average home user) and have a nice range of colors depending on what they're bound to. They are also comparatively easy to obtain and use (a 0.1% solution in H2O or 70% Ethanol or similar). You can also try Calcofluor, which stains cellulose and chitin. Its just going to be a bit harder to obtain (your DAPI cube will work well for the Calcofluor, and the FITC cube for the Eosin, as you would expect).

David