More diatoms, desmids and barnacles.
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More diatoms, desmids and barnacles.
20130505-DSLR_IMG_0212.jpg by pwnell, on Flickr
Stanley park ocean - diatoms on algae, 40x, FLUO-C4, HF C
20130505-DSLR_IMG_0227.jpg by pwnell, on Flickr
Stanley park ocean - barnacle, 10x, FLUO-C4
20130505-DSLR_IMG_0228.jpg by pwnell, on Flickr
Stanley park ocean - barnacle, 10x, FLUO-C4
20130508-DSLR_IMG_0124.jpg by pwnell, on Flickr
GTF water sample - Euastrum desmid,40x, DIC+QWP, HF B
20130508-DSLR_IMG_0261.jpg by pwnell, on Flickr
GTF water sample - diatom, 40x, DIC, HF B
20130508-DSLR_IMG_0274.jpg by pwnell, on Flickr
GTF water sample - diatom, 40x, DIC+QWP, ZS DMap
20130508-DSLR_IMG_0396.jpg by pwnell, on Flickr
GTF water sample - Euastrum desmid, 60x, QWP+LP, HF B
- arturoag75
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- Charles Krebs
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Correct - just refers to the cube's excitation/emission spectrum. Everything I do is auto fluorescence.discomorphella wrote:I really like the fluorescence shots. The barnacles are spectacular. I am assuming that you're not actually using DAPI or other fluorochromes but rather are referring to the labels on your cubes (the dye(s) your cubes were intended for) and these are autofluorescence images.
David
Thanks for the comments.
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Autofluorescence is great. Its amazing how well you can delineate all kinds of structures without any dye at all, and you have surely set the bar for those images, these are just great. For those specimens that are not so inclined, one thing you could try for less "glowy" subjects is Eosin Y or Phloxine B or similar dyes (all chemical relatives of Fluorescein). The fluorescence yield is good, and they are nontoxic (as compared to nasty DNA intercalators DAPI or Propidium, not for the average home user) and have a nice range of colors depending on what they're bound to. They are also comparatively easy to obtain and use (a 0.1% solution in H2O or 70% Ethanol or similar). You can also try Calcofluor, which stains cellulose and chitin. Its just going to be a bit harder to obtain (your DAPI cube will work well for the Calcofluor, and the FITC cube for the Eosin, as you would expect).
David
David