Thank you all for your kind comments.
Olympusman wrote:What is the dilution you are using for glutaraldehyde?
You need to use the lowest glutaraldehyde concentration possible. I normally work with 2% - 4%. In this case the fixation solution was 2,5% glutaraldehyde in cacodylate buffer (ph 7.2). The osmium tetroxide was 2% in aqua dest and was intended to harden the membranes.
Curt wrote:What's the scale of this photo?
This is a crop of the original 4000x magnification image. The crop spans 45 µm from left to right.
Betty wrote:Nice! What's the surface they are attached to? Is that SEM located at FU, HU, TU, some other institution, or do we have reason to turn green with envy as it's your personal toy?
The surface is a cover slip. This image was made with the SEM at the Freie Universität Berlin across the street from the botanical garden. You better prepare your family for the green colored Betty - I plan to get my own one in September.
Regards,
Ecki