Dividing Arcella, Frontonia, Paramecium pellicle, Holospora

Images made through a microscope. All subject types.

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Psi Wavefunction
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Dividing Arcella, Frontonia, Paramecium pellicle, Holospora

Post by Psi Wavefunction »

Back with a couple goodies!
And news: off to Halifax to start a MSc on protist diversity and cell structure in June; among my first tasks will be to bring the scope up to sexy standards. And then learn some TEM! (are electron micrographs allowed here, by the way?)

A dividing arcellinid (Arcella sp. here): the new shell is the clear one; thecae tend to "rust" over time and turn brown.

Frontonia full of tasty diatoms!

The giant Bursaria are notable for rapidly deciliating ciliates, like Paramecia, upon contact -- and completely by the time the ciliates pass the gullet. Bursarias also have this annoying habit of exploding when you look at them, thus releasing half-digested and not-yet-digested Paramecia into the surroundings. Conveniently enough, those Paramecia are free of cilia, so you can get a really nice crisp view of the pellicle!

Paramecium with a sporulating Holospora infection, from culture. The large refractile structure is... no, not the macronucleus. It is a micronucleus severely bloated by bacterial spores. Holospora spend part of their lives in nuclei, feeding on the chromatin and later sporulating. It's just one example of bacterial endosymbionts flourishing inside protist cells; not all are parasitic either.

Incidentally, I found a clump of Peritrichs with bacteria in the macronucleus (some dividing; arrowhead); this has apparently not been recorded in the literature before, and I lost the critters before getting around to culture them =(

Ok, this is probably enough to slow down the loading time. More later!

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Post by pwnell »

Very interesting, thanks.

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Post by Ecki »

Hi Psi,

Great to read you here! I enjoyed your blog a lot. SEM/TEM images are always welcome (I showed one today). In fact, I will be posting a lot of SEM images myself in a couple of months.

The Holospora infection is very interesting. In one of my amoeba cultures I found all amoeba with a strange "Nebenkörper" in the nucleus.

http://www.photomacrography.net/forum/v ... hp?t=13471

Do you have any idea what this could be?

Regards from Berlin,

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Re: Dividing Arcella, Frontonia, Paramecium pellicle, Holosp

Post by rjlittlefield »

Psi Wavefunction wrote:And then learn some TEM! (are electron micrographs allowed here, by the way?)
Just to confirm -- yes, absolutely. I have a particular interest in comparisons of optical & electron micrographs, to help us understand what can and cannot be seen with optical. Most readers will never have a chance to work directly with electron scopes, so it's nice to know what we're missing. :D


Bruce Taylor
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Post by Bruce Taylor »

Ecki wrote: I enjoyed your blog a lot.
Since you're using the past tense, you must be referring to Skeptic Wonder (not dead, apparently...merely encysted :) ). In case you've missed it, here is her "new" blog: http://blogs.scientificamerican.com/ocelloid/

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Post by RogelioMoreno »

Very interesting!

I could id the Apocarchesium thanks to your blog. :D


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Post by carlos.uruguay »

Very interesting! Thank you for sharing!!!

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