Carpenter Ant with Mitutoyo 2x, 10x, and 50x (images added)

Images made through a microscope. All subject types.

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curt0909
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Carpenter Ant with Mitutoyo 2x, 10x, and 50x (images added)

Post by curt0909 »

First test with a Mitutoyo 2x Planapo. 77 photos. 150mm mamiya used as tube lens which I don't recommend. I'll try again with a 200mm lens, hopefully tonight. All in all I'm happy with the quality of the center but the corners were poor and suffered from 'star' artifacts with stacking. The first image was heavily cropped (approx 2500x2500) to subject of interest. The second is actual pixels. I always feel weird posting these macro images in the microscope section but it was mounted on a vertical microscope set up. Image

100% crop
Image
Last edited by curt0909 on Fri Apr 26, 2013 4:29 pm, edited 8 times in total.

Jacek
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Post by Jacek »

Super

curt0909
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Post by curt0909 »

I took more photos of this ant today with the Mitutoyo 10x paired with Nikkor 200mm f4. 181 photos. I'm very happy with the performance of these objectives. I may try the 20 and 50x later and see what other details are hiding here. Unfortunately my lighting and composition skills are non existant but I'm a work in progress.

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100% crops Image

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Just for fun the black and white SEM lookImage
Last edited by curt0909 on Fri Apr 26, 2013 4:29 pm, edited 2 times in total.

Marek Mis
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Post by Marek Mis »

Very good and interesting images !

Marek

curt0909
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50x

Post by curt0909 »

I jumped the 20x and went straight for the 50x Mitutoyo. Two images. First shot with Mamiya 150mm giving approx 37.5x (54 photos)and second with an old 200mm Nikkor f4 for 50x (31 photos). 100% crops with each photo. Stacking these compound eyes is a real pain at this magnification.

Image

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Last edited by curt0909 on Fri Apr 26, 2013 4:32 pm, edited 3 times in total.

curt0909
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Post by curt0909 »

Thanks Marek!

canonian
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Re: 50x

Post by canonian »

curt0909 wrote:...First shot with Mamiya 150mm giving approx 37.5x and second with an old 200mm Nikkor f4 for 50x. 100% crops with each photo.
What a beautiful shot curt0909, I love the 37.5X photo.
It's so hard to keep up with the ever increasing quality of this forum.

curt0909
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Post by curt0909 »

Thank you Fred

Litonotus
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Post by Litonotus »

really great!

PS. how many images were in the 50x objective stack...? I'm just curious.
my FB page

I'm looking for the the extemely rare V-IM magnification changer for the E800 scope. If you have seen a listing or have one for sale please let me know.

curt0909
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Post by curt0909 »

Thanks Litonotus. I've just added the number of photos to my posts. The first 50x was 54 photos, second 31 photos.

Lothar-Gutjahr
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insanely jealous

Post by Lothar-Gutjahr »

Hello Curt,

Wow, what a nice toy, your lenses. You should keep them in a safe, grin.
May i ask about the tubelength you´r using ? Or do you cascade those lenses mentioned? Your imaging workflow would be interesting too, but i dont ask. Have a nice weekend !

Lothar

curt0909
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Post by curt0909 »

Hi Lothar,
The Mitutoyos and hands down the best epi objectives I've used. These are infintie objectives so magnification is not calculated by tubelength but by tube lens. Tube lenses were 150 or 200mm. I'm happy to tell my workflow. Its pretty basic.

Stack with Zerene Pmax and retouch as needed. I've found images shot with the 50x benefit more from pyramid stacking. The first 50x photo was pyramid stacked the second was not. I've actually restacked the second photo with the pyramid method and prefer the results of the 2nd version. Elf and Chris S. have made programs (ZereneVS and Slabber Jockey) to automate the process but I'm unable to use them so I did this manually. The stacking was as follows. 31 images.
First the photos were stacked in blocks of 4 with 2 overlap on each side. 1-4, 3-6, 5-8, 7-10, etc.
2 of the resulting images were stacked with 1 overlap on each side.
1-2, 2-3, 3-4, 4-5, etc
3 of these resulting iamges were stacked with 1 overlap on each side
1-3, 3-5, 5-7, 7-9 etc.
3 of the reusltoing images stacked 1 overlap on each side
1-3, 3-5
The final two images stacked. Its actually beneficial to do this manually in this case since most sub stacks needed retouching.

After this I open in Photoshop. First process is smart sharpen to pull some more detail from the image. After that I always use the same 4 effects to tailor the image.
-Color filters set to blue(make sure preserve luminosity is selected)
-Color filters set to green(make sure preserve luminosity is selected)
-Curves
-Vibrance and Saturation
Finally I run Topaz denoise to clean it up a bit

Here is the restacked version of the 2nd 50x photo. I wasn't happy with the color so I desaturated it and later colorized it.

Image

Image

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Last edited by curt0909 on Sat Apr 27, 2013 8:35 am, edited 1 time in total.

rjlittlefield
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Post by rjlittlefield »

Nice images!
curt0909 wrote:I've found images shot with the 50x benefit more from pyramid stacking. The first 50x photo was pyramid stacked the second was not. I've actually restacked the second photo with the pyramid method and prefer the results of the 2nd version. Elf and Chris S. have made programs (ZereneVS and Slabber Jockey) to automate the process but I'm unable to use them so I did this manually.
I think you're describing the process that we've come to call "slabbing".

I was initially confused by "pyramid stacking" because that phrase normally refers to stacking methods like PMax ("Pyramid Maximum") that internally operate on a pyramid of resized images. Google search on "pyramid methods in image processing" returns some good discussions.

--Rik

curt0909
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Post by curt0909 »

Thanks Rik,
And thanks for the correction. Its interesting to know that they used a similar process to create the first synthetic lifeform. Maybe Craig Venter has been lurking here. :lol:

'From here, the team devised a five stage assembly process where the cassettes were joined together in subassemblies to make larger and larger pieces that would eventually be combined to build the whole synthetic M. genitalium genome.'

http://www.jcvi.org/cms/research/%20pro ... s-release/

Lothar-Gutjahr
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Post by Lothar-Gutjahr »

Thanks Curt and Rik,

for me as a beginner in stacking it starts getting interesting how more variations like the mentioned slabbing is used. I did not understand when it is time to use it. This happens easy when your "life time waranty" is run out. Learning by doing is much better in my case. But what to do,grin.

Thank you both for your comments.

Lothar

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