







Moderators: rjlittlefield, ChrisR, Chris S., Pau
1,2,4,5 are DIC with Olympus 20x LUCPLANFL RC2 on Reichert Diastar with DIC condenser. I removed the 1.40 NA top lens and put a .90 NA top lens and use the 40x DIC prism with this objective. The RC2 is a relief contrast objective and not intended for DIC but I think it works well. Its got an added benefit of correction collar from 0-2mm cover slip.Charles Krebs wrote: Are you using oblique polarized brightfield here? (especially on last three).