Paramecium, bright field and phase

Images made through a microscope. All subject types.

Moderators: rjlittlefield, ChrisR, Chris S., Pau

Posts: 438
Joined: Sat Aug 05, 2006 8:34 am
Location: Germany, Duesseldorf

Paramecium, bright field and phase

Post by ralfwagner »


these are my first shots of a paramecium that really show some details.

You can see the 2 contractile vacuoles encircled by their feeding ducts, the oral groove with the formation of a new food vacuole at its end and the overall surrounding cilia.


The next phase-contrast picture lets us have a look at the macronucleus (red arrow). It is a dark oval partially hidden by a contractile vacuole. The yellow arrow in the inset points to what I am thinking of is the micronucleus. Does somebody know if that could be true?


In the first two pictures it was necessary to have the paramecium in a rather flat state under the coverslip (actually it died a few seconds after my last shot). Otherwise the shown details would have been unvisible. On the other hand it is interesting to show the spacial dimensions of that critter, too. This picture of a freely mobile and unflattened paramecium shows us that especially the oral zone is completely different now.


Charles Krebs
Posts: 5865
Joined: Tue Aug 01, 2006 8:02 pm
Location: Issaquah, WA USA

Post by Charles Krebs »

I particularly like the first shot as it shows important features that might normally take several shots to illustrate. You have 2 nice wev, the formation of a new food vacuole is clearly shown, and the cilia are nicely represented as well.

Posts: 3578
Joined: Fri Aug 04, 2006 4:19 am
Location: Southern New Hampshire USA

Post by beetleman »

A Very detailed little critter Ralf. Excellent work :wink:
Take Nothing but Pictures--Leave Nothing but Footprints.
Doug Breda

Ken Ramos
Posts: 7208
Joined: Thu Jul 27, 2006 2:12 pm
Location: lat=35.4005&lon=-81.9841

Post by Ken Ramos »

Nice shots there Ralf. :D As for the micronuclei, I would have no idea. Most of the time I have a hard time finding the macronuclueus unless it is well defined in the subject, however, I do see what you are referring too :wink: .

Post Reply Previous topicNext topic