Butterfly scales under fluorescent excitation

Images made through a microscope. All subject types.

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pwnell
Posts: 2029
Joined: Fri Dec 18, 2009 4:59 pm
Location: Tsawwassen, Canada

Butterfly scales under fluorescent excitation

Post by pwnell »

I was surprised to see the colours (except for the green) to mimic the actual colours of the scales. Red is now orange and black stays black. Cannot recall what butterfly this was - it was from the Papilionidae genus.

Image
20130202-DSLR_IMG_0486.jpg by pwnell, on Flickr
Butterfly scales, 10x, FLUO-C4, ZS PMax

emsiem
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Location: Zagreb, Croatia
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Post by emsiem »

Is this autofluorescence? What kind of excitation light you used?
Love the colors!
Nenad

pwnell
Posts: 2029
Joined: Fri Dec 18, 2009 4:59 pm
Location: Tsawwassen, Canada

Post by pwnell »

Yep, autofluorescence. I used a 330 - 480nm excitation source.

RogelioMoreno
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Joined: Fri Nov 20, 2009 11:24 am
Location: Panama

Post by RogelioMoreno »

Interesting!

Rogelio

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