Xanthidium armatum, LM and SEM

Images made through a microscope. All subject types.

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Gerd
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Location: Duesseldorf, Germany

Xanthidium armatum, LM and SEM

Post by Gerd »

Reading Eckis posting about SEM (http://www.photomacrography.net/forum/v ... hp?t=19666)
i tried a much easier subject to prepare, an empty shell of an Desmid, Xanthidium armatum.
These are very stable shells, you can find them in "forgotten" pond samples..
No need for any fixation or critical point drying.
With a Pipette i took some of the shells out of an old sample and washed them in alcohol. After drying up i transfered the shells to a Sem stub.
After Gold sputtering, we examined the sample in Sem.
For comparison, here is a LM pic with the typical mucilage sheath.

Image

This is the SEM view, note the several small holes, i think these are the pores where the mucilage is ejected.

Image


Thanks for looking,
Gerd

Jacek
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Post by Jacek »

impressive :shock:

Ecki
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Post by Ecki »

Dear Gerd,

this is indeed a very nice image. For me the most amazing feature of the SEM besides resolution and magnification is the depth of field compared to the light microscope. When you have seen the true three dimensional structure of a protozoan, light microscopic images make much more sense - it is like "now you know what you see".

Lately I have been working with Klaus on some pretty amazing stuff - you will see images soon.

Best regards and happy eastern also to your wife,
Ecki

rjlittlefield
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Post by rjlittlefield »

Wonderful images!
For me the most amazing feature of the SEM besides resolution and magnification is the depth of field compared to the light microscope.
Yes, the short wavelength of electrons permits stopping down a long ways. Here we're seeing the difference between f/1 and f/1000 or so.

--Rik

Wim van Egmond
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Post by Wim van Egmond »

Ah, superb! Very nice to see the threedimensional shape and the fine details.

Wim

RogelioMoreno
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Post by RogelioMoreno »

Very nice, the depth of field and details is amazing.

Rogelio

Pau
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Post by Pau »

A most interesting comparison!
Thanks for sharing these nice images.
Pau

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