Water Snail embryo

Anymo2001 · Post by **Anymo2001** » Mon Mar 25, 2013 3:19 pm

Hello everybody,
I am new in this forum. I have been reading posts here for a long time, and I have enjoyed and learnt a lot with all your suggestions, and images (specially fron Charles Krebs, but also others).
I am now adding a movie of a water snail egg with the embryo moving inside, which I think it is a begining of my sharing. I hope this is the first example of a long list.

The movie is taken using a Nikon D800 camera through a Nikon optiphot-2 equipped with a polarizing set. I used brightfield, darkfield, polarized and combination of styrene filters combined in a P.A.C.O device. 4x, 10x and 10x plan acromatic objectives.

I hope you liked, it is a bit long...
Best regards,
Antonio

http://m.youtube.com/watch?v=Z6cAz8GSsJw&feature=plcp

curt0909 · Post by **curt0909** » Tue Mar 26, 2013 6:30 pm

Great video. I like all the color variations

Anymo2001 · Post by **Anymo2001** » Thu Mar 28, 2013 3:02 pm

Thanks, the system is quite flexible.

Charles Krebs · Post by **Charles Krebs** » Thu Mar 28, 2013 6:28 pm

Greetings Antonio,

You certainly did run through some lighting variations in that video. I found myself most interested in observing the specimen during the darkfield sections. Good old darkfield seems hard to beat for a subject like this.

Anymo2001 · Post by **Anymo2001** » Fri Mar 29, 2013 1:22 am

Thanks Charles,
I agree, that darkfield here is the best lighting system.
I also tried to follow the developing process of the embryo with darkfield as the sample gets less dry with time, however it is a long process and finally I could not get it, just first cell divisions, may be with time I will learn how to do it.
Best regards,
Antonio