Closterium in Interphako

Frank Fox · Post by **Frank Fox** » Mon Mar 11, 2013 8:11 am

hello,

I'll show you photos of Closterium in contrast "Interphako" by Zeiss Jena.
More photos are on my website.
http://www.mikro-foto.de/closterium-interphako.html

Pictures of the Interphako microscope you can see here:
http://www.mikro-foto.de/Equipment.html

Cactusdave · Post by **Cactusdave** » Mon Mar 11, 2013 8:52 am

Very interesting form of contrast enhancement. I have never seen effects quite like this, and it is both visually informative and rather beautiful. The name "Interphako" (and perhaps the appearance of the images themselves) gives the idea that this is some combination of phase and differential interference contrast. Is that correct?

curt0909 · Post by **curt0909** » Mon Mar 11, 2013 10:25 am

Excellent photos. Frank I've been looking through your site for the last few months and I'm very impressed with your photos. Top notch quality

arturoag75 · Post by **arturoag75** » Mon Mar 11, 2013 11:57 am

Superb shots and impressive equipment

Arturo

Jacek · Post by **Jacek** » Mon Mar 11, 2013 11:57 am

Super !

interesting technique

Litonotus · Post by **Litonotus** » Mon Mar 11, 2013 12:55 pm

great images from great microscope. unusual techique perfect for diatoms, I would say sometimes better tahn DIC.

Frank Fox · Post by **Frank Fox** » Tue Mar 12, 2013 4:57 am

Thanks all.

Interphako - method:
By a Mach-Zehnder interferometer, the beam is split imaging side (important).
In one of the two beam paths is now by a suitable baffle arrangement (phase rings) the object retained on the diffracted light, that is the
Object structures are filtered out. If this beam is transmitted only a plane wave.
Both partial beams are reunited, the distorted wave interferes with the plane wave.

That would be a rough description of the procedure.

I will show even diatoms in Interphako here.

curt0909 · Post by **curt0909** » Tue Mar 12, 2013 5:48 am

Thanks for the explanation Frank. I was way off.

I found a diagram of the light path of an Interphako microscope. Page 119

http://books.google.com/books?id=r8T7zJ ... ko&f=false

Cactusdave · Post by **Cactusdave** » Tue Mar 12, 2013 7:26 am

Thanks for that explanation Frank. You are most fortunate to have access to such a fine instrument. I am deeply envious !

harisA · Post by **harisA** » Wed Mar 13, 2013 10:31 am

Amazing photos. Zeiss jena CF250 objectives were among the best at those times.

RogelioMoreno · Post by **RogelioMoreno** » Fri Mar 15, 2013 6:42 am

Frank,

Very nice details!

Thank you for the explanation.

Rogelio