Large Acanthocystis

Linden.g · Post by **Linden.g** » Wed Mar 06, 2013 7:11 pm

This example was larger than I have seen before, first image taken with 40x S-plan achromat and second two with 100x using 2.5x NFK projective photoeyepiece on Olympus BH-2 with DIC flash. I also never noticed how the glass spines meet so neatly in the center of the sphere as seen in the last image.

Jacek · Post by **Jacek** » Thu Mar 07, 2013 2:27 am

excellent details

carlos.uruguay · Post by **carlos.uruguay** » Thu Mar 07, 2013 4:54 am

Beautiful images!
carlos

Bernd · Post by **Bernd** » Thu Mar 07, 2013 10:41 am

Excellent photos!

Just for clarification: The glass spines are attached to the surface of the heliozoon. It is the axiopodia that meet in the center of the sphere.

Bernd

Linden.g · Post by **Linden.g** » Thu Mar 07, 2013 3:11 pm

Bernd wrote:Excellent photos!

Just for clarification: The glass spines are attached to the surface of the heliozoon. It is the axiopodia that meet in the center of the sphere.

Bernd

Thanks for the clarification, so the axiopodia are acting like muscle fibers moving the glass spines? are they all attached together in the center?

Charles Krebs · Post by **Charles Krebs** » Thu Mar 07, 2013 3:40 pm

Very nice!

Rarely (if ever) have I seen the shapes at the ends of the spines as well as you capture them here.

Linden.g · Post by **Linden.g** » Thu Mar 07, 2013 6:21 pm

Charles Krebs wrote:Very nice!

Rarely (if ever) have I seen the shapes at the ends of the spines as well as you capture them here.

Thanks Charlie, I think the organsms size helped. I corrected the terminology for the 2.5X NFK lens thanks for the pointer, it's always appreciated.

arturoag75 · Post by **arturoag75** » Fri Mar 08, 2013 1:07 am

Excellent shots!

Ichthyophthirius · Post by **Ichthyophthirius** » Fri Mar 08, 2013 4:25 am

Excellent shots of this heliozoon!

I am also astonished by the quality of the image. I can't see any colour fringes; is this normal for Olympus Planachromats or have you applied some image processing to remove the fringes?

Kind regards,

Jon

Bruce Taylor · Post by **Bruce Taylor** » Fri Mar 08, 2013 6:48 am

Dazzlingly clear view of the scales!

Ferry · Post by **Ferry** » Fri Mar 08, 2013 12:37 pm

Really amazing!
Concerning the spines in the third image, these are new spines. They will be transported to the cell surface.

myriophyllum · Post by **myriophyllum** » Fri Mar 08, 2013 12:38 pm

Hi,

great, i've newer seen such a good shot of the centromer
like your last picture!

Best regards

Jens

Linden.g · Post by **Linden.g** » Fri Mar 08, 2013 2:39 pm

Ichthyophthirius wrote:Excellent shots of this heliozoon!

I am also astonished by the quality of the image. I can't see any colour fringes; is this normal for Olympus Planachromats or have you applied some image processing to remove the fringes?

Kind regards,

Jon

Hi Jon. I've taken the first image as shot, with all its blemishes and warts, as it came out of the camera as a RAW file and shown it below. All I did was to reduce it in size/bit depth so I could post it. I also took a segment from the center and pasted it in the corner. As you can see there is significant CA. I reduce CA in photoshop and make the most of the available data. RAW files often need substancial work, and optical performance isn't always achieved as in this case with a mid range objective and a deep slide.

Ichthyophthirius · Post by **Ichthyophthirius** » Fri Mar 08, 2013 3:18 pm

Hi Linden. Thanks for these explanations. As I said, the results are quite spectacular.

Jon