in the first part of my Amphipleura posts (http://www.photomacrography.net/forum/v ... hp?t=19501) I presented some images using darkfield.
Let me now show the best resolution I could reach.
Again I took my Leuchtbildkondensor (nA 1.5) and stopped down an objektiv 63/1.4
to about 1.2 asymmetrically by a black paper.
Two crossed polars improved contrast.
First an image using white light:
sections out of that image:
blue light, 450 nm; filter 450DF50:
sections out of that image:
blue light again, but focussed slightly deeper:
sections out of that image:
I think the different look of the outer and inner side of the shell produces that effect.
For a comparation: on the sites of Peter H. You can find this very fine image:
http://www.mikroskopie-ph.de/Amphi-FESEM-09.jpg
finally, I have some distances:
Zeiss Standard 18, Planapo 63/1.4; S-Kpl 16x/12.5; 63mm relay lens; DSLR
I collected the diatoms at the Kraterquelle of Bad Nenndorf, Germany.
Naphrax was used for embedding.
Your comments are welcome!
Greetings
Jens
