Marine organisms under fluorescent excitation

pwnell · Post by **pwnell** » Thu Jan 17, 2013 8:56 pm

I do apologise if I am posting too many photos, but I have been extremely excited about my fluorescence cube I recently received.

Below are some marine crustaceans and other organisms, all under various fluorescence excitations.

20130115-DSLR_IMG_0150.jpg by pwnell, on Flickr
Flatworm, 10x, FLUO-C4

20130116-DSLR_IMG_0069.jpg by pwnell, on Flickr
Amphipod, 4x, FLUO-C4

20130116-DSLR_IMG_0109.jpg by pwnell, on Flickr
Copepod, 20x, FLUO-C3, HF C

20130116-DSLR_IMG_0216.jpg by pwnell, on Flickr
Isopod, 10x, FLUO-C4

20130116-DSLR_IMG_0232.jpg by pwnell, on Flickr
Copepod, 20x, FLUO-C4, HF C

20130116-DSLR_IMG_0397.jpg by pwnell, on Flickr
Copepod, 40x, FLUO-C4

20130116-DSLR_IMG_0505.jpg by pwnell, on Flickr
Diatom, 40x, FLUO-C4

Jacek · Post by **Jacek** » Thu Jan 17, 2013 11:00 pm

beautiful

RogelioMoreno · Post by **RogelioMoreno** » Fri Jan 18, 2013 2:00 am

Beautiful set!

Rogelio

arturoag75 · Post by **arturoag75** » Fri Jan 18, 2013 2:13 am

once again super job

Starshade · Post by **Starshade** » Fri Jan 18, 2013 3:58 am

Wow! THat's impressive. Could you please tell more about Fluo-C4? (As well as C3) Simple search doesn't give me any results. Is that a set of filters?

curt0909 · Post by **curt0909** » Fri Jan 18, 2013 7:11 am

Very nice

pwnell · Post by **pwnell** » Fri Jan 18, 2013 10:04 am

Starshade wrote:Wow! THat's impressive. Could you please tell more about Fluo-C4? (As well as C3) Simple search doesn't give me any results. Is that a set of filters?

Thanks everyone. It is my own codes to describe what I used. Fluo = Fluorescence. C3 is a GFP cube. C4 is a custom cube I had made, with a broad band UV/blue light excitation filter, and a long pass 488nm emission filter.

Charles Krebs · Post by **Charles Krebs** » Fri Jan 18, 2013 2:56 pm

Definitely impressive! Fascinating.

Starshade · Post by **Starshade** » Fri Jan 18, 2013 4:31 pm

Thanks everyone. It is my own codes to describe what I used. Fluo = Fluorescence. C3 is a GFP cube. C4 is a custom cube I had made, with a broad band UV/blue light excitation filter, and a long pass 488nm emission filter.

Thanks Waldo,

Could you also post (or pm) a link to a spectrogram of these cubes if they are somewhere in the net? I am curious because I had a very similar cube to your c3 and it never gave me any cool results with autofluorescence. Admittedly, I didn't have marine organisms to try it on. Marine creatures are designed to glow in uv/deep blue and during my uv dives I often would look at glowing plankton too - the smallest guys were the brightest among invertebrates.

pwnell · Post by **pwnell** » Fri Jan 18, 2013 6:49 pm

Starshade wrote:Could you also post (or pm) a link to a spectrogram of these cubes if they are somewhere in the net?

Sent pm.

Frank · Post by **Frank** » Fri Jan 18, 2013 8:25 pm

Your results are remarkable. Are these wet mounts and do you use a coverslip? I have an old AO flourescent scope that I have never used. I
'd like to dust it off and give it a go. I have two filters for it I'll have to see how close the frequencies are to what you are usiing.

Frank

pwnell · Post by **pwnell** » Fri Jan 18, 2013 11:31 pm

Frank wrote:Your results are remarkable. Are these wet mounts and do you use a coverslip? I have an old AO flourescent scope that I have never used. I
'd like to dust it off and give it a go. I have two filters for it I'll have to see how close the frequencies are to what you are usiing.

Frank

Yep, wet mounts and with coverslip.

naturephoto1 · Post by **naturephoto1** » Sun Jan 20, 2013 5:03 pm

Waldo,

Excellent, well shot, and outstanding images.

Rich