GFP Fluorescence in Green Star Polyps

Images made through a microscope. All subject types.

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pwnell
Posts: 2031
Joined: Fri Dec 18, 2009 4:59 pm
Location: Tsawwassen, Canada

GFP Fluorescence in Green Star Polyps

Post by pwnell »

So I just received my Semrock GFP filter cube and tried my hand at fluorescence photomacrography for the first time. Feedback is appreciated.

First the polyp under normal transmitted illumination (10x)

Image
20121116-DSLR_IMG_0012.jpg by pwnell, on Flickr

Now for my hand at fluorescence.

Image
20121228-DSLR_IMG_0017.jpg by pwnell, on Flickr
Green star polyp, 10x, GFP FLUO

Image
20121228-DSLR_IMG_0015.jpg by pwnell, on Flickr
Green star polyp, 4x, GFP FLUO

Image
20121228-DSLR_IMG_0024.jpg by pwnell, on Flickr
Green star polyp, 40x, GFP FLUO

harisA
Posts: 519
Joined: Sun Jul 03, 2011 9:31 am
Location: Greece

Post by harisA »

Nice images. I also want to play with fluorescence photomacrography. How easy is to find gfp (dye) and at what price?How you apply it at your specimen?
Regards

Haris

pwnell
Posts: 2031
Joined: Fri Dec 18, 2009 4:59 pm
Location: Tsawwassen, Canada

Post by pwnell »

I did not. The GFP is natural in the tissue of this auto fluorescence coral.

g4lab
Posts: 1461
Joined: Fri May 23, 2008 11:07 am

Post by g4lab »

I have some GFP and other fluorescent protein filter sets as well as filter sets for some other fluorochromes. These are generally sized for Zeiss P&C reflector cubes, but probably could fit some other makes too.

I also have various light sources for fluorescence available, including HBO housings and power supplies and also some of the more recent Osram HXP based units from Exfo (EXFO X-Cite).

If anyone is interested send me a private message. Prices would what only could be described as "small fractional"

You can probably buy some animals (live) which have been gene spliced to make GFP and other FPs online.

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