Difflugia

Images made through a microscope. All subject types.

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Charles Krebs
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Difflugia

Post by Charles Krebs »

A current marsh sample had a huge number of these testate amoebae. Two of the images (1 and 3) were photographed on a wet mount slide on the Olympus BHS using polarized light.

Then I tried something to get a little different "look" to the tests. They were placed on a slide with no water or cover slip, and photographed on the MM-11 microscope arrangement used for "dry" subjects (#2 and 4). Lighting was from above using two Ikea LED lamps through large ping-pong ball diffusers, with things set up to give a darkfield type of illumination. The tests I gathered measured about 300x180 microns (0.012x0.007 inches) in size.


Olympus BHS, Olympus S Plan Apo 10/0.40. Brightfield with partially crossed polarizers. 20X on sensor. 14 image stack. (Image cropped)
Image


Nikon MM-11. Olympus LMPLFLN 50/0.50. Reflected light. 50X on sensor. 85 image stack.

Image

Olympus BHS, Olympus S Plan Apo 4/0.16. Brightfield with partially crossed polarizers. 8X on sensor. 11 image stack.
Image

Nikon MM-11. Mitutoyo M Plan Apo 10/0.28. Reflected light. 9X on sensor. 24 image stack.

Image

curt0909
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Post by curt0909 »

In this case epi-illumination looks much better than transmitted in my opinion. Those are the best photos of a testate ameoba I've seen.

Litonotus
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Post by Litonotus »

curt0909 wrote: Those are the best photos of a testate ameoba I've seen.
I agree.

Wim van Egmond
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Post by Wim van Egmond »

Very nice to see this subject photograhed this way. I wonder how testae amoes like Arcella looks with this magnification. Or diatoms.

Wim

Ecki
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Post by Ecki »

Very nice indeed. I prefer the reflected light images, too. How did you arrange the shells so neatly?

arturoag75
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Post by arturoag75 »

Absolutely outstanding the second one..fantastic! :shock: :wink:
arturo

Jacek
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Post by Jacek »

As always, the sheer beauty

Charles Krebs
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Post by Charles Krebs »

Thanks for the kind comments!

Ecki...
Very nice indeed. I prefer the reflected light images, too. How did you arrange the shells so neatly?
Under a stereo microscope using a very thin insect mounting pin and a great deal of patience :wink: . I actual started off with a larger number in the group but lost a few during the arranging process.

I was particularly happy the way the he 50X shot turned out (I've tried this a couple of times before with less success). The ability to control the light so that it accentuates the texture of the test is a big advantage over "normal" diascopic or episcopic microscope lighting. When the test is completely dry it does not photograph that well. It is similar to the way a dry shell, or piece of dry beach-glass takes on a "frosted" appearance. They look better wet. But too much water causes very troublesome reflections from the light source. This time the tests were immersed in a mixture of glycerin and water. When removed from the solution, the water eventually evaporated but a thin coating of glycerin stayed on the tests and provided the "wet" look.

Wim van Egmond
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Post by Wim van Egmond »

Very clever how you manipulated the humidity. For manipulating shells like this you could also try a cat's whisker. They lose them regularly so you often find them in the house. If you don't have a cat ask a friend who has one. The tip is very thin and the hair is strong. If the tip is to thin you can cut a part of it at the desired thickness. Where would we be without cats.

Wim

RogelioMoreno
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Post by RogelioMoreno »

Very nice and the idea to keep them wet is amazing!

Rogelio

carlos.uruguay
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Post by carlos.uruguay »

A marvel!!
carlos

Ecki
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Post by Ecki »

Charles,

Thanks for the explanation.

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