Mayfly nymph

Images made through a microscope. All subject types.

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Charles Krebs
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Mayfly nymph

Post by Charles Krebs »

1, 2, 3, 5 and 6 are (partially) crossed polarizers in brightfield. 4 and 7 :smt119 are DIC.

With these I find the respiratory system and "tails" most interesting.

Olympus BHS, Olympus S Plan Apo 4/0.16. Brightfield with partially crossed polarizers. 22 image stack.
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Olympus BHS, Olympus S Plan Apo 4/0.16. Brightfield with partially crossed polarizers. 12 image stack.
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Olympus BHS, Olympus S Plan Apo 10/0.40. Brightfield with partially crossed polarizers. 20 image stack.
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Olympus BHS, Olympus S Plan Apo 10/0.40. DIC illumination. 23 image stack.
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Olympus BHS, Olympus S Plan Apo 10/0.40. Brightfield with partially crossed polarizers. 35 image stack.
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Olympus BHS, Olympus S Plan Apo 10/0.40. Brightfield with partially crossed polarizers. 28 image stack.
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Olympus BHS, Olympus S Plan Apo 10/0.40. DIC illumination (DIsney Chrome in this case :wink:). 28 image stack.
Image
Last edited by Charles Krebs on Thu Nov 29, 2012 11:47 am, edited 1 time in total.

curt0909
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Post by curt0909 »

All great. My favorites are the first and last.

pierre
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Post by pierre »

A top work !

Impressive view of the internals.

Thanks for the share.
Regards

Pierre

Jacek
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Post by Jacek »

Great series, you are unbeatable

Cactusdave
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Post by Cactusdave »

Great work, I love the first image. Are these stacked or is the depth due to judicious use of the aperture diaphragm? There is a quality of sharpness and solidity in these images and those from Jacek, that I much admire and envy. :)
Leitz Ortholux 1, Zeiss standard, Nikon Diaphot inverted, Canon photographic gear

Planapo
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Post by Planapo »

Lovely!
As I have a better understanding and a little practical experience 8) of how DIC is set-up and working now :wink: :D , I'd think that you've stacked the last one with the upper prism moved between single shots?

--Betty
Atticus Finch: "You never really understand a person until you consider things from his point of view
- until you climb into his skin and walk around in it."
Lee, N. H. 1960. To Kill a Mockingbird. J. B. Lippincott, New York.

Franz Neidl
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Post by Franz Neidl »

I like very much the first picture. It is looking like an extraterrestrial animal, maybe coming from Mars (not yet discovered from "Curiosity").

My questions: What objective did you use for the first picture: probably 4x,
but planapo, apo , fluor, achromatic ecc. ? It is a stack with how many pictures?

Franz

canonian
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Post by canonian »

Wonderful, Charles.
You keep raising the bar and you are a great inspiration to us all.
About the last one: a real winner for next months pm.net frontpage.

RogelioMoreno
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Post by RogelioMoreno »

Beautiful set!

Rogelio

abpho
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Post by abpho »

Simply amazing.
I'm in Canada! Isn't that weird?

Ecki
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Post by Ecki »

Very nice.The depth of field of the first DIC image is amazing.

Wim van Egmond
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Post by Wim van Egmond »

Beautiful! But that is not surprising. :)

Wim

Charles Krebs
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Post by Charles Krebs »

Thanks all for the kind remarks!

Dave and Franz....
I've gone back and added some info to each picture. The first two are with the Olympus 4/0.16 S Plan Apo; and all are from "stacks".

Betty...
I'd think that you've stacked the last one with the upper prism moved between single shots?
Good guess, and I wish that were the case :cry:. Unfortunately this is the result of a troubling aspects of my DIC set-up. With the 10X is it very difficult to get a homogenous background, both in terms of color and density. With a "grey" background I can tweak the DIC settings to get a useable result. But when the slider is dialed up into the "colors" it is hard to avoid a multi-color background. In this shot I just decided to "use it" since it made an interesting result with the geometry of the subject.

While the polarizers and DIC make for interesting results with the subject in the last three shots, I think I actually prefer the results from "plain old" brightfield and darkfield done a while ago (see what you think...):
http://www.photomacrography.net/forum/v ... php?t=8400

fpelectronica
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Post by fpelectronica »

! Very very nice !
Francisco

carlos.uruguay
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Post by carlos.uruguay »

Magical images!
carlos

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