Crustaceans

Images made through a microscope. All subject types.

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pwnell
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Crustaceans

Post by pwnell »

Image
Amphipod, 4x * 1.6, Polarised

Image
Marine copepod, 40x * 1.6, Polarised

Image
Head of an isopod, 40x * 1.25, Polarised + Lambda plate, 40 image stack using Helicon Focus C.

Fredlab
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Post by Fredlab »

Hello

The last one is incredible.
The grey mosaïc : do you think is it cells ?
I apologise for my poor english
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pwnell
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Post by pwnell »

Nope I'd rather suggest it is some crystallisation in its exoskeleton. The pattern is too precise, too angular to be cells.

Fredlab
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Post by Fredlab »

OK... you are right, it looks like a (micro)quartzite
(link)
(but cuticule is organic)
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pwnell
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Post by pwnell »

Yep makes sense, as I know these exoskeletons are sometimes consumed when the organisms molt.

Jacek
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Post by Jacek »

great photos

Winger
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Post by Winger »

Wow...wonderful photos. I love it!

arturoag75
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Post by arturoag75 »

Last shot is impressive :shock:

Chris S.
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Post by Chris S. »

Waldo, you've lately posted quite a few lovely images that I've much enjoyed. But your "Marine copepod, 40x * 1.6, Polarised" photograph is one that I keep returning to, and find especially wonderful. Bravo!

I do wonder what you mean by "* 1.6"--does this indicate the relay lens you used? And by "Polarised"--do you mean that you placed a polarizing filter on your light, placed an analyzer (aka "polarizer") behind your objective, both of the above (cross-polarization) or something else?

Cheers,

--Chris

pwnell
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Post by pwnell »

Chris S. wrote:Waldo, you've lately posted quite a few lovely images that I've much enjoyed. But your "Marine copepod, 40x * 1.6, Polarised" photograph is one that I keep returning to, and find especially wonderful. Bravo!
Thank you for your kind words.
Chris S. wrote:I do wonder what you mean by "* 1.6"--does this indicate the relay lens you used?
I have a magnification changer on my microscope, so this means I had it set to 1.6 times magnification, hence the image on the film plane (sorry, CCD plane) are not that from the 40x/0.95 objective alone, but has been magnified 1.6 times. I find that with low power objectives one get usable additional resolution by using the magnification changer. At 40x the advantage goes away - there is not much more resolution to be had on the 18MP sensor by enlarging the image circle, at least not visually for me. So in this case it was mostly used for framing. Call me old school but I'd rather get the framing as I want it optically, rather than reverting to cropping in post.
Chris S. wrote:And by "Polarised"--do you mean that you placed a polarizing filter on your light, placed an analyzer (aka "polarizer") behind your objective, both of the above (cross-polarization) or something else?
By polarised I mean that there is a polariser below the condenser, and an analyser above the objectives (the same configuration used for DIC). The analyser and polariser is at 90 degrees to each other, therefore the background light is extinct (black) and I get maximum polarisation. I basically just slide out the objective and condenser Nomarski prisms.

Chris S.
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Post by Chris S. »

Thanks for the additional explanation, Waldo. It's very clear, now.

Can't blame you for preferring to crop in camera, even if the added magnification is empty--I'd no doubt make the same decision. A wise friend of mine says that it you waste beer, you go to hell; I believe this is also true of pixels.

--Chris

Charles Krebs
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Post by Charles Krebs »

Waldo,

Very nice images!

The patterns that are revealed using polarized light with these creatures are quite graphic and interesting. Did some a while ago....
http://www.photomacrography.net/forum/v ... php?t=3182

... but always wanted to explore them more. These images have rekindled my interest.

Pau
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Post by Pau »

Waldo, I have seen this same crystal pattern in the shell of freshwater ostracods and cladocerans, I thinked them may be calcium carbonate because here the waters are very rich in calcium.
Pau

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