Urtica by Warhol

Images made through a microscope. All subject types.

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Fredlab
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Joined: Fri Nov 09, 2012 11:23 am
Location: Burgundy
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Urtica by Warhol

Post by Fredlab »

Hello

Few weeks ago, i bought a DIC condenser for my BH2.
This technology is really amazing

Image

Enjoy !
I apologise for my poor english
My blog (Macro Micro World)
My gallery

Pau
Site Admin
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Joined: Wed Jan 20, 2010 8:57 am
Location: Valencia, Spain

Post by Pau »

Fred, wellcome!

The images doesm't seem true DIC but polarized light with retarders. Do you match the condenser prisms with the objective and the DIC slider?
Pau

Fredlab
Posts: 304
Joined: Fri Nov 09, 2012 11:23 am
Location: Burgundy
Contact:

Post by Fredlab »

Hello

Oh, i think my condenser is fully fonctionnal, but for these shots, i play with the upper prism.

Another shot, not in "zazu" mode

Image

but, i'm a beginner in microphoto, DIC and some tecs...

Lower prisms are made for SPlan, for these shots, i use Leitz objective because i haven't SPlan... I just received two SPlan (10 an 40) I think it will make easier some adjustments

Thanks for your com'
I apologise for my poor english
My blog (Macro Micro World)
My gallery

Pau
Site Admin
Posts: 5717
Joined: Wed Jan 20, 2010 8:57 am
Location: Valencia, Spain

Post by Pau »

Yes, this one is good DIC!
Pau

Cactusdave
Posts: 1631
Joined: Tue Jun 09, 2009 12:40 pm
Location: Bromley, Kent, UK

Post by Cactusdave »

Clearly DIC is working OK in the new posting. I just assumed that the first posting involved taking pictures with increased rotation or displacement of the DIC De Senarmont compensator or waveplate, with the top left image showing more or less 'normal' grey background DIC displacement of the compensator and displacement increasing from left to right and top to bottom, producing an 'optical staining' effect. There is an explanation of this effect here http://en.wikipedia.org/wiki/Differenti ... microscopy

Quote from this source:
'The contrast can be adjusted using the offset phase, either by translating the objective Normarski prism, or by a lambda/4 waveplate between polarizer and the condenser Normarski prism (De-Senarmont Compensation). The resulting contrast is going from dark-field for zero phase offset (intensity proportional to the square of the shear differential), to the typical relief seen for phase of ~5–90 degrees, to optical staining at 360 degrees, where the extinguished wavelength shifts with the phase differential.'

The effect on the image as the phase offset is varied with the compensator from 0 degrees to 360 degrees is illustrated here http://en.wikipedia.org/wiki/File:DIC_Phase.jpg
Leitz Ortholux 1, Zeiss standard, Nikon Diaphot inverted, Canon photographic gear

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