Epi Illumination Comparison

curt0909 · Post by **curt0909** » Wed Oct 31, 2012 4:29 am

A comparison of epi illumination methods on an insect. I did not include the classic diffused DF, only settings from the microscope (Reichert Epistar with DIC option). I didn't put too much time into this, stacks are incomplete, no post processing. I know I've attached more than the allowed number of photos but I was hoping you could let it slide this time.

Insect eye - 60x 0.8
DF

Pol

DIC

DIC color

Insect anterior thorax 10x 0.25
DF

BF

Pol

DIC

fpelectronica · Post by **fpelectronica** » Wed Oct 31, 2012 5:53 am

Very interesting
Francisco

Pau · Post by **Pau** » Fri Nov 02, 2012 10:03 am

Iteresting comparison, thanks for posting it.
Unfortunately reflected DIC seems of little utility with biological specimes as we previoully discussed
http://www.photomacrography.net/forum/v ... 301#109301

curt0909 · Post by **curt0909** » Fri Nov 02, 2012 11:03 am

I agree, there are a few biological specimens that respond well to DIC. In these cases DIC can help determine topography. Other than these few cases I prefer diffused darkfield.