unknown small testate amoebae

[jc maccagno]

40X 100X objectiv




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[Ferry]

It is impossible to say even which genus it is when I can't see it in side view. I always tap on the cover slip to get it off the glass to see it in lateral view. Now there are to many possibilities.

Cheers,

Ferry

[jc maccagno]

Thank you for your interest

I will try your tip!

John

[Ferry]

The smaller, the more difficult it is to get them off the glass. It often fails...

[Olympusman]

Ferry,
That's a great resource for amoebae. I am working off some old and new pond dips and finding hundreds of tests in a single drop, but none that are occupied. Do you know if methyl cellulose has an adverse effect on amoebae?

[Ferry]

Well, I have never used that, but I don't think it has any effect on empty test, but I'm sure you can't use it for living amoebae. But why should you use it? To slow down any movement? Or to manipulate some tests e.g. for looking into the aperture or to see it in lateral view? I use glycerol for that purposes...

[Olympusman]

I'm just getting back into pond water and I'm trying to do focus stacks and the MC keeps the desmids such as Closterium from moving around too much between shots. I was wondering about the effect on amoebae because I was able to shoot a sequence of a rather large rotifer that appeared to have suffocated from the MC -- it slowed down and its V shaped feet started slowing down to where I could get shots of the feet opened. However, on the rotifer I didn't do a stack, I just used a smaller condenser aperture to yield greater depth of field.