Pond Water Life Forms

flyer2o12 · Post by **flyer2o12** » Wed Oct 03, 2012 11:56 am

My first images from work. The polarised images were acquired with the Nikon Eclipse 80i and the fluorescent images were acquired with the Zeiss Axioimager.

PS. Sorry for the poor resolution but the images are quite high resolution and the site does not permit high resolution images so they had to be significantly compressed.

This image below shows auto-fluorescence of Daphnia irradiated by 358 nm (blue emission), 488 nm (green emission) and 650 nm (red emission).

sebba28 · Post by **sebba28** » Wed Oct 03, 2012 12:30 pm

Beautiful images.

Cactusdave · Post by **Cactusdave** » Wed Oct 03, 2012 1:09 pm

Great images. It's always good fun when you can play with a super lab microscope like this, and the pictures are likely to be top notch.

Pau · Post by **Pau** » Thu Oct 04, 2012 2:26 am

Nice images!, I most like the last one. Did you use the 60D or the microscope camera?

The last one, is true fluorescence? I ask this because the excitation colors seem to be the same as the image colors. As I understand, for exemple, if you use blue excitation light the emision light will be of longer wavelengh and the barrier filter would cut the blue light.

flyer2o12 · Post by **flyer2o12** » Thu Oct 04, 2012 5:32 am

Pau, this is the natural auto-fluorescence of Daphnia. I chose blue for the Dapi channel, green for the GFP channel and Red for Cy5 channel, though you can choose any colours you like, so yes these are the true auto-fluoresence colours.

I used the axiocam for these images on the zeiss axioimager microscope.

Marek Mis · Post by **Marek Mis** » Thu Oct 04, 2012 6:02 am

Nice images.
Your microscope is a real beast ! I'm waiting for more photographs in verious techniques.

Marek