Pond Water Life Forms

Images made through a microscope. All subject types.

Moderators: rjlittlefield, ChrisR, Chris S., Pau

flyer2o12
Posts: 223
Joined: Mon Jan 09, 2012 5:06 pm
Location: Ireland
Contact:

Pond Water Life Forms

Post by flyer2o12 »

My first images from work. The polarised images were acquired with the Nikon Eclipse 80i and the fluorescent images were acquired with the Zeiss Axioimager.

PS. Sorry for the poor resolution but the images are quite high resolution and the site does not permit high resolution images so they had to be significantly compressed.

Image

Image


Image

This image below shows auto-fluorescence of Daphnia irradiated by 358 nm (blue emission), 488 nm (green emission) and 650 nm (red emission).
Image

sebba28
Posts: 301
Joined: Thu Dec 29, 2011 11:13 pm
Location: Poland,Ireland

Post by sebba28 »

Beautiful images.

Cactusdave
Posts: 1631
Joined: Tue Jun 09, 2009 12:40 pm
Location: Bromley, Kent, UK

Post by Cactusdave »

Great images. It's always good fun when you can play with a super lab microscope like this, and the pictures are likely to be top notch.
Leitz Ortholux 1, Zeiss standard, Nikon Diaphot inverted, Canon photographic gear

Pau
Site Admin
Posts: 6120
Joined: Wed Jan 20, 2010 8:57 am
Location: Valencia, Spain

Post by Pau »

Nice images!, I most like the last one. Did you use the 60D or the microscope camera?

The last one, is true fluorescence? I ask this because the excitation colors seem to be the same as the image colors. As I understand, for exemple, if you use blue excitation light the emision light will be of longer wavelengh and the barrier filter would cut the blue light.
Pau

flyer2o12
Posts: 223
Joined: Mon Jan 09, 2012 5:06 pm
Location: Ireland
Contact:

Post by flyer2o12 »

Pau, this is the natural auto-fluorescence of Daphnia. I chose blue for the Dapi channel, green for the GFP channel and Red for Cy5 channel, though you can choose any colours you like, so yes these are the true auto-fluoresence colours.

I used the axiocam for these images on the zeiss axioimager microscope.

Image

Marek Mis
Posts: 2586
Joined: Sat Jul 10, 2010 9:56 am
Location: Suwalki, Poland
Contact:

Post by Marek Mis »

Nice images.
Your microscope is a real beast ! I'm waiting for more photographs in verious techniques.

Marek

Post Reply Previous topicNext topic